| Project/Area Number |
24510321
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
RUMIANA Bakalova 独立行政法人放射線医学総合研究所, 分子イメージング研究センター, 主任研究員 (30469936)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Ichio (ZHELEV Zhivko) 独立行政法人放射線医学総合研究所, 分子イメージング研究センター, チームリーダー (10319519)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | Polymersomes / Quantum dots / Nitroxides / Drug delivery systems / Cancer / 量子ドッド / ニトロキシラジカル / DDS / 蛍光イメージング / in vivo イメージング / 腫瘍 / MRI / drug-delivery systems / nanoparticles / polymersomes / quantum dot / nitroxides / fluorescent imaging / EPR imaging / cancer / ニトロキシルラジカル / レドックス / 酸化還元 / 量子ドット / センサー / 蛍光 / 反応性造影剤 |
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| Outline of Final Research Achievements |
We developed polymersomes, embedding with quantum dots (QDs) and hydrophobic nitroxide-labeled anticancer drug, as a new sensing platform for imaging of drug delivery in cancer. If both substances are retained into the polymersome, there is no fluorescence of QDs. If nitroxide-labeled drug is released from polymersomes, the fluorescence of QDs appeared.
On experimental cancer-bearing animals, using optical imaging, we established that these nanoparticles were appropriate for imaging of cancer, lymph node mapping, as well as for imaging of drug delivery. A strong fluorescent signal, corresponding to QD705 fluorescence, was detected in the tumor, as well as into the lymph nodes. The half-life of QD/nitroxide-embeding polymersomes was 6±2 hours in the bloodstream, 8±2 hours into the tumor and 11±3 hours in the lymph nodes. The data suggests these size-controlled long-circulating polymersomes are useful matrix for development of nano-formulations with theranostic capabilities.
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