Photo-induced stractural dynamics of proteins studied with intense mid-infrared laser pulses
| Project/Area Number |
24540323
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Condensed matter physics I
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 超高速分光 / タンパク質 / 反応制御 |
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| Outline of Final Research Achievements |
To reveal proton transfer and structural isomerization dynamics of photoactive yellow protein (PYP), we have developed the pump-pump-probe spectroscopy: The first pump pulse at 400 nm initiates the photocycle of PYP, the second pulse at mid-infrared modulates the vibrational states of the chromophore, and the subsequent broadband probe pulse measures the absorption spectrum of PYP. We have examined the effect of the mid-infrared pump pulse on the photocycle of PYP with a variety of the frequency and timing of the second pump pulse. It is found that the effect is less than our system noise level, 10<sup>-4</sup>. However, by using this method, we obtained the unified view of the vibrational energy flow in the electronic ground state of PYP. In addition, ultrafast hydrogen bonding dynamics in the electronic excited state was revealed with the femtosecond stimulated Raman spectroscopy.
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Report
(4 results)
Research Products
(13 results)
