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Nano scale in vivo imaging of contractile proteins in mouse skeletal muscle

Research Project

Project/Area Number 24540430
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics/Chemical physics
Research InstitutionThe University of Tokyo

Principal Investigator

KAYA Motoshi  東京大学, 理学(系)研究科(研究院), 助教 (00422098)

Co-Investigator(Renkei-kenkyūsha) KOBAYASHI Takuya  東京大学, 大学院総合文化研究科, 研究員 (60468585)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords筋肉 / サルコメア / in vivoイメージング / 生物物理
Outline of Final Research Achievements

In muscle, myosins are contractile protein that interacts with and pulls actin filaments toward the center of muscle. The recent single molecule studies have revealed various properties of muscle myosins, however it still remains unknown that how function of individual myosins is integrated into its molecular ensembles in sarcomere, which is an contractile unitary structure. In order to investigate whether they are simply a sum of their functional units or they enhance the functional output in their molecular ensembles, the experimental system was successfully developed to detect dynamics of single actin filaments in sarcomere structures by injecting DNA of the target protein, tropomodulin, into mouse skeletal muscles. Tropomodulin is located at the tip of actin filament and can be labeled by fluorescent particles (e.g., quantum dots) so that individual actin filaments can be visualized and are trackable,

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (5 results) (of which Invited: 5 results) Book (1 results)

  • [Journal Article] Stiffness, working stroke and force of single myosins in skeletal muscle: Elucidation of these mechanical properties by non-linear elasticity2013

    • Author(s)
      Kaya. M. and H. Higuchi
    • Journal Title

      Cell and Mol. life Sci

      Volume: 70 Issue: 22 Pages: 4275-4292

    • DOI

      10.1007/s00018-013-1353-x

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prediction of muscle forces using static optimization for different contractile conditions.2013

    • Author(s)
      Arslam Y. Z., Jinha A., Kaya M. and Herzog W.
    • Journal Title

      Journal of Mechanics in Medicine and Biology

      Volume: 13

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Response to Letter to the Editor regarding Jinha et al. (2009) "A task-specific validation of homogeneous non-linear optimization approaches’’2012

    • Author(s)
      Jinha A., Ait-Haddou R., Kaya M. and Herzog W.
    • Journal Title

      Journal of Theoretical Biology

      Volume: 306 Pages: 145-145

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 筋肉の巧みな収縮メカニズム.2012

    • Author(s)
      茅 元司,樋口秀男
    • Journal Title

      感染・炎症・免疫

      Volume: 42 Pages: 116-123

    • Related Report
      2012 Research-status Report
  • [Presentation] 1分子顕微鏡を用いて見えてきた筋肉の効率的な収縮メカニズム2014

    • Author(s)
      茅 元司
    • Organizer
      日本光学会年次学術講演会シンポジウム「バイオフォトニクスの展望」
    • Place of Presentation
      筑波大学東京キャンパス文京校舎
    • Year and Date
      2014-11-05 – 2014-11-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 1分子計測技術を用いて効率的な筋収縮の仕組みを紐解く2014

    • Author(s)
      茅 元司
    • Organizer
      第87回日本生化学会大会シンポジウム「次世代型筋研究の夜明け」
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 骨格筋ミオシン分子複合体の力発生に特化したミオシン1 分子の特性とダイナミクス2013

    • Author(s)
      茅 元司, 樋口 秀男
    • Organizer
      第 51 回日本生物物理学会年会
    • Place of Presentation
      国立京都国際館
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Molecular mechanism of muscle contraction - Elucidation from single molecule approaches -2013

    • Author(s)
      茅 元司
    • Organizer
      マルチスケール骨格筋収縮動態に関する国際シンポジウム
    • Place of Presentation
      神奈川大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 分子複合フィラメントにおける骨格筋ミオシン1分子の力発生メカニズム.2012

    • Author(s)
      茅 元司
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 1分子生物学」原田慶恵・石渡信一編2014

    • Author(s)
      茅 元司
    • Total Pages
      292
    • Publisher
      化学同人
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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