The study of light-induced structural change and DNA binding formation in Aureochrome with NMR
| Project/Area Number |
24540435
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics/Chemical physics
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| Research Institution | Osaka University |
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Principal Investigator |
HAMADA Norio 大阪大学, 産学連携本部, 講師 (80379148)
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| Co-Investigator(Kenkyū-buntansha) |
HISATOMI Osamu 大阪大学, 大学院理学研究科, 准教授 (60231544)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | NMR / 構造変化 / 光受容タンパク質 / DNA結合 / 転写因子 / 光構造変化 / タンパク質 / タンパク質間相互作用 / 光制御 |
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| Outline of Final Research Achievements |
Aureochrome-1 is a blue light receptor that mediated the branching response in stramenopile alga and light-regulated transcription factor, although molecular mechanism is unknown. We have tried to measure light-induced structural change and DNA binding formation in Aureochrome with NMR spectroscopy. Light-induced structural change signals were observed in photo-steady state of Aureochrome with NMR. We confirmed the different peaks between dark state and photo-steady state in Aureochrome. However some peaks were not assigned in photo-steady state due to signal broadening. To obtain the structural information, we produced mutants according to similar LOV domain sequences. We found that some mutants were stabilized photo-steady conformation.
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Report
(4 results)
Research Products
(12 results)
