| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Outline of Final Research Achievements |
Aureochrome-1 is a blue light receptor that mediated the branching response in stramenopile alga and light-regulated transcription factor, although molecular mechanism is unknown. We have tried to measure light-induced structural change and DNA binding formation in Aureochrome with NMR spectroscopy. Light-induced structural change signals were observed in photo-steady state of Aureochrome with NMR. We confirmed the different peaks between dark state and photo-steady state in Aureochrome. However some peaks were not assigned in photo-steady state due to signal broadening. To obtain the structural information, we produced mutants according to similar LOV domain sequences. We found that some mutants were stabilized photo-steady conformation.
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