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The study of light-induced structural change and DNA binding formation in Aureochrome with NMR

Research Project

Project/Area Number 24540435
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics/Chemical physics
Research InstitutionOsaka University

Principal Investigator

HAMADA Norio  大阪大学, 産学連携本部, 講師 (80379148)

Co-Investigator(Kenkyū-buntansha) HISATOMI Osamu  大阪大学, 大学院理学研究科, 准教授 (60231544)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
KeywordsNMR / 構造変化 / 光受容タンパク質 / DNA結合 / 転写因子 / 光構造変化 / タンパク質 / タンパク質間相互作用 / 光制御
Outline of Final Research Achievements

Aureochrome-1 is a blue light receptor that mediated the branching response in stramenopile alga and light-regulated transcription factor, although molecular mechanism is unknown. We have tried to measure light-induced structural change and DNA binding formation in Aureochrome with NMR spectroscopy. Light-induced structural change signals were observed in photo-steady state of Aureochrome with NMR. We confirmed the different peaks between dark state and photo-steady state in Aureochrome. However some peaks were not assigned in photo-steady state due to signal broadening. To obtain the structural information, we produced mutants according to similar LOV domain sequences. We found that some mutants were stabilized photo-steady conformation.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Molecular mechanism of Photozipper, a light-regulated dimerizing module consisting of the bZIP and LOV domains of Aureochrome-12015

    • Author(s)
      Y. Nakatani, O. Hisatomi
    • Journal Title

      Biochemistry

      Volume: E-pub Issue: 21 Pages: 10-10

    • DOI

      10.1021/acs.biochem.5b00320

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blue light-induced dimerization of monomeric aureochrome-1 enhances its affinity for the target sequence.2014

    • Author(s)
      O. Hisatomi, Y. Nakatani, K. Takahashi, H. Kataoka
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 25 Pages: 17379-17391

    • DOI

      10.1074/jbc.m114.554618

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Accuracy of Protein Size Estimates Based on Light Scattering Measurements.2014

    • Author(s)
      Takeuchi, K. , Nakatani, Y. and Hisatomi, O
    • Journal Title

      Open Journal of Biophysics

      Volume: 4 Pages: 83-91

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular Mechanism of a Photoregulated bZIP Transcription Factor and Its Application as a Molecular Tool2014

    • Author(s)
      久冨 修
    • Journal Title

      Seibutsu Butsuri

      Volume: 54 Issue: 6 Pages: 307-310

    • DOI

      10.2142/biophys.54.307

    • NAID

      130004713030

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blue Light-Induced Conformational Change in a Light-Regulated Transcription Factor, Aureochrome-12013

    • Author(s)
      Osamu Hisatomi, Ken Takeuchi, Kazunori Zukihara, Yuki Ookubo, Yoichi Nakatani, Fumio Takahashi, Satoru Tokutomi and Hironari Kataoka
    • Journal Title

      Plant & Cell Physiol

      Volume: 54(1) Issue: 12 Pages: 93-106

    • DOI

      10.1093/pcp/pcp160

    • NAID

      10031145653

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ultrafast Hydrogen-Bonding Dynamics in the Electronic Excited State of Photoactive Yellow Protein Revealed by Femtosecond Stimulated Raman Spectroscopy2012

    • Author(s)
      中村亮介
    • Journal Title

      Journal of Physical Chemistry B

      Volume: 116 Issue: 51 Pages: 14768-14775

    • DOI

      10.1021/jp308433a

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] A photo-activated basic-leucine zipper module, opZL2014

    • Author(s)
      O. Hisatomi
    • Organizer
      16th ICRP (International Conference on Retinal Protein
    • Place of Presentation
      Nagahama, Japan
    • Year and Date
      2014-10-05 – 2014-10-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Influence of steric limitations around chromophore in protein cage of photoactive yellow protein2014

    • Author(s)
      N. Hamada, N. Inazumi, R. Nakamura
    • Organizer
      16th ICRP (International Conference on Retinal Protein
    • Place of Presentation
      Nagahama, Japan
    • Year and Date
      2014-10-05 – 2014-10-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 光受容タンパク質の光誘起構造変化と水素結合ダイナミクス2014

    • Author(s)
      中村亮介、濱田格雄
    • Organizer
      第69回日本物理学会
    • Place of Presentation
      東海大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Functional analyses of each domain in Aureochrome-12013

    • Author(s)
      中谷 陽一, 竹内 健 , 井澤 洋介 , 高橋 文雄, 片岡 博尚, 久冨 修
    • Organizer
      第51回生物物理学会
    • Place of Presentation
      京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Measurements of molecular weights of soluble proteins using static light scattering2013

    • Author(s)
      竹内 健, 中谷 陽一, 久冨 修
    • Organizer
      第51回生物物理学会
    • Place of Presentation
      京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] 青色光により励起される転写因子AUREO1の構造変化2012

    • Author(s)
      Osamu Hisatomi, Ken Takeuchi,,Yoichi Nakatani, Fumio Takahashi,Hironari Kataoka
    • Organizer
      生物物理学会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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