Development of visulization system of proteins adsorbed at interfaces

• Project/Area Number: 24540444
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biophysics/Chemical physics
• Research Institution: Kinki University
• Principal Investigator: YANO Yohko 近畿大学, 理工学部, 准教授 (70255264)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: Ｘ線反射率 / Ｘ線回折 / FT-IR / タンパク質 / 界面吸着 / X線反射率法 / 界面 / Ｘ線反射率法 / たんぱく質 / 固液界面 / 赤外吸収分光法

Outline of Final Research Achievements

We have constructed an energy dispersive X-ray reflectometer combined with ATR-FTIR system. The reflectivity data were obtained with high resolution even
by use of a conventional x-ray tube. The reflectometer equipped achieved x-ray reflectivity towards 10-5 with the integration time of only 10 sec. We have also succeeded to perform X-ray diffraction and FT-IR simultaneously. having enormous potential for various measurements.

Research Products

• [Journal Article] Real-time investigation of protein unfolding at an air-water interface at the 1 s time scale (2013)
  • Author(s): Yohko F. Yano, Etsuo Arakawa, Wolfgang Voegeli, and Tadashi Matsushita
  • Journal Title: Journal of synchrotron radiation Volume: 20 Issue: 6 Pages: 980-983
  • DOI: 10.1107/s0909049513023741
  • Peer Reviewed
• [Journal Article] Effect of salt ions on protein layers at the air-water interface under a crystallization condition (2013)
  • Author(s): YF Yano, T Uruga
  • Journal Title: Chemical Physics Volume: 419 Pages: 153-155
  • DOI: 10.1016/j.chemphys.2013.02.018
  • Peer Reviewed
• [Journal Article] A simultaneous multiple angle-wavelength dispersive X-ray reflectometer using a bent-twisted polychromator crystal (2013)
  • Author(s): Tadashi Matsushita, Etsuo Arakawa, Wolfgang Voegeli, Yohko F Yano
  • Journal Title: Journal of synchrotron radiation Volume: 20 Issue: 1 Pages: 80-88
  • DOI: 10.1107/s0909049512043415
  • Peer Reviewed
• [Journal Article] Effect of salt ions on protein layers at the air-water interface under a crystallization condition (2013)
  • Author(s): Yohko F Yano, Tomoya Uruga
  • Journal Title: Chemical Physics Volume: 416
  • Peer Reviewed
• [Journal Article] Kinetics of protein unfolding at interfaces (2012)
  • Author(s): Yohko F. Yano
  • Journal Title: Journal of Physics: Condensed Matter Volume: 24 Issue: 50 Pages: 503101-503101
  • DOI: 10.1088/0953-8984/24/50/503101
  • Peer Reviewed
• [Presentation] Protein unfolding at an air-water interface investigated by a simultaneous multiple angle-wavelength dispersive x-ray reflectometer (2013)
  • Author(s): Yohko F. Yano, Etsuo Arakawa, Wolfgang Voegeli, and Tadashi Matsushita
  • Organizer: 回折構造生物国際シンポジウム2013
  • Place of Presentation: 名古屋市中小企業振興会館（愛知）
• [Presentation] 液体表面で見られるタンパク質の塩析現象(4) (2013)
  • Author(s): 矢野 陽子・新田 清文・宇留賀 朋哉
  • Organizer: 第36回溶液化学シンポジウム
  • Place of Presentation: 北海道大学（北海道）
• [Presentation] Protein Salting out Observed at an Air-water Interface (2013)
  • Author(s): Y. F. Yano, K. Nitta, and T. Uruga
  • Organizer: 33rdInternational Conference on Solution Chemistry
  • Place of Presentation: 京都テレサ（京都）
• [Presentation] X-ray reflectivity from liquid surfaces (2013)
  • Author(s): Yohko F. Yano, Etsuo Arakawa, Wolfgang Voegeli, and Tadashi Matsushita
  • Organizer: 2013 JSAP-MRS Joint Symposia
  • Place of Presentation: 同志社大学（京都）
• [Presentation] X線反射率法によるタンパク質の界面吸着ダイナミクスの観測 V: ミリ秒計測を目指した測定系の飛躍的迅速化 (2012)
  • Author(s): 矢野陽子, 荒川悦雄, Voegeli Wolfgang, 松下正, 宇留賀朋哉
  • Organizer: 第5回分子科学討論会
  • Place of Presentation: 東京大学（東京都）
• [Presentation] 液体表面で見られるタンパク質の塩析現象(3) (2012)
  • Author(s): 矢野陽子、宇留賀 朋哉
  • Organizer: 第35回溶液化学シンポジウム
  • Place of Presentation: 早稲田大学（東京都）
• [Presentation] Kinetics of protein unfolding at interfaces (2012)
  • Author(s): Yohko F. Yano
  • Organizer: 12th International Conference on Surface X-ray and Neutron Scattering
  • Place of Presentation: Kolkata（インド）
• [Book] 放射光が拓く化学の現在と未来: 物質科学にイノベーションをもたらす光 (2014)
  • Author(s): 日本化学会 編
  • Total Pages: 161
  • Publisher: 化学同人
• [Remarks] 生物物理学研究室 研究内容
  • URL: http://qube.phys.kindai.ac.jp/users/yano/index.html