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Laser Ionization of Biologically Active Substances Using Zeolites

Research Project

Project/Area Number 24550030
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Physical chemistry
Research InstitutionTokyo Metropolitan University

Principal Investigator

TATSUYA Fujino  首都大学東京, 理工学研究科, 准教授 (20360638)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsレーザーイオン化法 / 酸化物固体表面 / ナノ微粒子 / 半導体 / 血清 / 固体酸化物表面 / 脂肪酸 / MALDI法 / ゼオライト / 生理活性物質 / レーザーイオン化
Outline of Final Research Achievements

Zeolites are crystalline aluminosilicates with nanometer-order cages. Zeolites have high catalytic activity due to the charge imbalance at the Si-O-Al bridging sites, and those sites are compensated typically by such cations as H+ and Na+. Hydroxyl (OH) groups having Brönsted acidity exist in H+-exchanged zeolite. It is well known that the Brönsted acid site is responsible for the various catalytic activities of zeolite. In our recent research, we discovered that zeolite was also applicable to mass spectrometric studies. We have developed a “zeolite matrix,” which is a complex of organic MALDI matrix and zeolite. We succeeded in observing large ion peaks of analyte. In addition, we found that zeolite matrix is applicable to biologically active substances, which cannot be detected by conventional MALDI. We are fully convinced that the zeolite matrix can further improve the applicability of MALDI-MS.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (32 results)

All 2014 2013 2012

All Journal Article (22 results) (of which Peer Reviewed: 22 results,  Acknowledgement Compliant: 4 results) Presentation (9 results) (of which Invited: 5 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Quantitative analysis of free fatty acids in human serum using biexciton Auger recombination in cadmium telluride nanoparticles loaded on zeolite2014

    • Author(s)
      M. Yang, T. Fujino
    • Journal Title

      Analytical Chemistry

      Volume: 86 Pages: 9563-9569

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Stabilization of matrix molecules by nonvolatile analyte plays an important role in matrix-assisted laser desorption ionization2014

    • Author(s)
      J Xu, T. Fujino
    • Journal Title

      Chemical Physics Letters

      Volume: 609 Pages: 6-10

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Gold nanoparticles loaded on zeolite as inorganic matrix for laser desorption/ionization mass spectrometry of low molecular weight compounds2014

    • Author(s)
      M. Yang, T. Fujino
    • Journal Title

      Chemical Physics Letters

      Volume: 592 Pages: 160-163

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cation-selective ionization of biologically active substances using zeolites2014

    • Author(s)
      T. Fujino, J. Suzuki, R. Yamamoto
    • Journal Title

      Science of Advanced Materials

      Volume: 6 Pages: 1590-1593

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of laser ionization techniques for evaluation of the effect of the cancer drugs using imaging mass spectrometry2014

    • Author(s)
      H. Kannen, H. Hazama, Y. Kaneda, T. Fujino, K. Awazu
    • Journal Title

      International Journal of Molecular Science

      Volume: 15 Pages: 11234-11244

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Investigation of ionization assisting effect of zeolite for a drug in imaging mass spectrometry using laser desorption/ionization2014

    • Author(s)
      H. Hazama, N. Moriguchi, T. Fujino, K. Awazu
    • Journal Title

      IEEJ Transactions on Electronics, Information and Systems

      Volume: 134 Pages: 657-663

    • NAID

      10031118920

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multi-element detection in green, black, oolong, and pu-erh teas by ICP-MS2014

    • Author(s)
      Q. Han, S. Mihara, T. Fujino
    • Journal Title

      Journal of Biochemistry and Physiology

      Volume: 3 Pages: 132-135

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optimization of tea sample preparation methods for ICP-MS and application to verification of Chinese tea authenticity2014

    • Author(s)
      Q. Han, S. Mihara, K. Hashimoto, T. Fujino
    • Journal Title

      Food Science and Technology Research

      Volume: 20 Pages: 1109-1119

    • NAID

      130004848126

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Femtosecond time-resolved laser desorption/ionization mass spectrometry of perylene crystals using induced Raman vibrational excitation2013

    • Author(s)
      Y. Minegishi, J. Matsumoto, H. Shiromaru, K. Hashimoto, *T. Fujino
    • Journal Title

      Chemical Physics Letters

      Volume: 584 Pages: 14-17

    • DOI

      10.1016/j.cplett.2013.08.055

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Silver nanoparticles on zeolite surface for laser desorption/ionization mass spectrometry of low molecular weight compounds2013

    • Author(s)
      M. Yang, *T. Fujino
    • Journal Title

      Chemical Physics Letters

      Volume: 576 Pages: 61-64

    • DOI

      10.1016/j.cplett.2013.05.030

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] SmFeN micro particles for matrix-assisted laser desorption/ionization mass spectrometry of model peptides2013

    • Author(s)
      Y. Ding, *T. Fujino
    • Journal Title

      Chemical Physics Letters

      Volume: 574 Pages: 116-119

    • DOI

      10.1016/j.cplett.2013.04.061

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Settlement of sweet-spot problem of MALDI crystals using cyclodextrin-supported matrix2013

    • Author(s)
      T. Fujita, *T. Fujino
    • Journal Title

      Chemistry Letters

      Volume: 42 Pages: 350-351

    • NAID

      10031162169

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Efficient laser desorption ionization mass spectrometry of polycyclic aromatic hydrocarbons using excitation energy transfer from anthracene2013

    • Author(s)
      K. Fujimori, *T. Fujino
    • Journal Title

      Chemical Physics

      Volume: 419 Pages: 97-100

    • DOI

      10.1016/j.chemphys.2013.02.014

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Study of ionization process of matrix molecules in matrix-assisted laser desorption ionization2013

    • Author(s)
      K. Murakami, A. Sato, K. Hashimoto, *T. Fujino
    • Journal Title

      Chemical Physics

      Volume: 419 Pages: 37-43

    • DOI

      10.1016/j.chemphys.2012.12.014

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cyclodextrin-supported organic matrix for application of MALDI-MS for forensics. Soft-ionization to obtain protonated molecules of low molecular weight compounds2013

    • Author(s)
      Tetsu Yonezawa, Takashi Asano, Tatsuya Fujino, Hiroshi Nishihara
    • Journal Title

      Chem. Phys.

      Volume: 419 Pages: 17-22

    • DOI

      10.1016/j.chemphys.2012.10.003

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Laser desorption ionization mass spectrometry of biological active substances using alkali cation-substituted zeolites2013

    • Author(s)
      T. Asano, J. Suzuki, K. Hashimoto, *T. Fujino
    • Journal Title

      Analytical Science

      Volume: 29 Pages: 1035-1039

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 松坂牛・飛騨牛の安定同位体比分析2013

    • Author(s)
      青柳寛司, 後藤晶子, 藤野竜也, 伊永隆史
    • Journal Title

      日本食品科学工学会誌

      Volume: 60 Pages: 138-141

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Matrix-assisted laser desorption ionization mass spectrometry of maltohexaose and acetylsalicylic acid using alkali metal cation-substituted zeolites2012

    • Author(s)
      J. Suzuki, T. Fujino
    • Journal Title

      Analytical Science

      Volume: 28 Pages: 901-904

    • NAID

      10031069406

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Matrix-assisted laser desorption ionization using lithium-substituted mordenite surface2012

    • Author(s)
      J. Suzuki, A. Sato, R. Yamamoto, T. Asano, T. Shimosato, H. Shima, J.N. Kondo, K. Yamashita, K. Hashimoto, T. Fujino
    • Journal Title

      Chemical Physics Letters

      Volume: 546 Pages: 159-163

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 2,4,6-trihydroxyacetoohenone on zeolite surface: correlation between electronic relaxation and fragmentation on mass spectra2012

    • Author(s)
      R. Yamamoto, T. Fujino
    • Journal Title

      Chemical Physics Letters

      Volume: 543 Pages: 76-81

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Desorption Dynamics of Tetracene Ion from Tetracene-doped Anthracene Crystals Studied by Femtosecond Time-Resolved Mass Spectrometry2012

    • Author(s)
      Y. Minegishi, D. Morimoto, J. Matsumoto, H. Shiromaru, K. Hashimoto, T. Fujino
    • Journal Title

      Journal of Physical Chemistry C

      Volume: 116 Pages: 3059-3064

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Methyl and ethyl chloroformate derivatizations for compound-specific stable isotope analysis of fatty acid-II2012

    • Author(s)
      K. Aoyagi, A. Yamanaka, A.S. Goto, T. Fujino, T. Korenaga, Y. Chikaraishi
    • Journal Title

      Res. Org. Geochem.

      Volume: 28 Pages: 27-30

    • NAID

      110009593003

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 半導体を用いた生理活性物質の定量分析2014

    • Author(s)
      藤野竜也
    • Organizer
      第2回レーザーイオン化研究会
    • Place of Presentation
      首都大学東京
    • Year and Date
      2014-11-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 半導体微粒子を用いたレーザーイオン化質量分析法による脂肪酸の定量分析2014

    • Author(s)
      M. Yang, 藤野竜也
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Application of MALDI-MS for Forensics; Mass Measurement of Low-molecular-weight Drugs Using Zeolite Matrix2014

    • Author(s)
      Tatsuya Fujino
    • Organizer
      Annual Conference and EXPO of AnalytiX-2014
    • Place of Presentation
      Dalian, China
    • Year and Date
      2014-04-25 – 2014-04-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Ionization and desorption process in marix-assisted laser desorption ionization studied by time-resolved mass spectrometry2014

    • Author(s)
      T. Fujino
    • Organizer
      8th Asian cinference on ultrafast phenomena
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Femtosecond time-resolved mass spectrometry for the desorption process of tetracene Ions from tetracene-doped anthracene crystals2013

    • Author(s)
      T. Fujino
    • Organizer
      International Conference and Exhibition on Analytical & Bioanalytical Techniques
    • Place of Presentation
      Las-Vegas, USA
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Laser desorption/ionization mass spectrometry of biologically active substances using zeolite surface2013

    • Author(s)
      T. Fujino, J. Suzuki, R. Yamamoto, T. Asano, and K. Hashimoto
    • Organizer
      Asian conference on analytical science (ASIANALYSIS XII)
    • Place of Presentation
      Fukuoka, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] Femtosecond Time-Resolved Mass Spectrometry for Desorption Dynamics of Tetracene Ion from Tetracene-doped Anthracene Crystals2012

    • Author(s)
      Y.Minagishi, D.Morimoto, J.Matsumoto, H.Shiromaru, K.Hashimoto, T.Fujino
    • Organizer
      19th International Mass Spectrometry Conference (IMSC2012)
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] Cation Selective Ionization of Biologically Active Substances Using Zeolites2012

    • Author(s)
      T.Fujino, J.Suzuki, R.Yamamoto
    • Organizer
      International Conference on Emerging Advanced Nanomaterials (ICEAN2012)
    • Place of Presentation
      ブリスベン(オーストラリア)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] マトリクス支援レーザー脱離イオン化(MALDI)法における 脱離イオン化過程の再考察2012

    • Author(s)
      村上雅和,藤野竜也,松本淳,城丸春夫,橋本健朗
    • Organizer
      分子科学討論会2012
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 分析用試薬組成及び分析方法2012

    • Inventor(s)
      藤野竜也
    • Industrial Property Rights Holder
      首都大学東京
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-197282
    • Filing Date
      2012-09-07
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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