| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Molecular dynamics in membrane inside and outside was quantified by multinuclear, dynamic NMR in situ, in relation to the thermal fluctuation of membranes. First, the lipid membrane dynamics was successfully characterized in cell sized vesicles (CSVs) of 10-20 μm-diameters by using solution-state NMR. The dynamic structure of CSVs was found to be different from nano-sized vesicles of ~100 nm-diameters frequently used. The contribution of membrane fluctuation to molecular transport was also quantified in the presence of various amounts of cholesterol in the membrane.
The peptide permeation to living cell inside was also observed by real-time in-cell NMR in situ and analyzed by first-order kinetics. Such kinetic approach was also applicable to time-resolved studies of biochemical reactions in peptides such as peptide bond cleavage in human-lens crystallin fragment in situ.
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