| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Outline of Final Research Achievements |
Green photosynthetic bacteria have unique antenna systems called chlorosomes. A themophilic bacterium Chlorobaculum tepidum strain biosynthesized MGDG and RGDG possessing a methylene-bridged palmitoleyl group characterized by a cis-substituted cyclopropane ring. The formation of the cyclopropane ring was chemically inhibited. The ring-formation represented direct and post-synthetic modifications of chlorosome membrane properties and was tolerant of acidic environments.
The glycolipids in various green bacteria were investigated. Dependence of the molecular structures including the chain-length of their acyl groups upon bacterial cultivation temperatures was clearly observed. The organisms with their optimal temperatures of 30, 45, and 55 ℃ dominantly accumulated glycolipids with the acyl chains in the range of C15-C16, C16-C17, and C18-C20, respectively. Based on the detailed of glycolipids, a survival strategy of green bacteria grown in the wide range of temperatures is proposed.
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