| Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In this study, to increase the detection sensitivity in capillary electrophoresis (CE) and microchip electrophoresis (MCE), a novel and simple on-line sample preconcentration based on a modified dynamic pH junction technique in a bipolar electrode embedded single “straight” channel was developed. It was found that a very high pH zone with a short band width formed around the bipolar electrode only by switching the polarity of the separation voltage applied both ends of the straight microchannel. As a typical result, a 50～300-fold increases in the sensitivity for fluorescent dyes were obtained. Proposed technique was successfully applied to the analysis of labeling dyes for biomolecules and the separation of protein mixtures.
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