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Development of the specific detection methods to identify the binding epitope of ligand interacting with the target protein

Research Project

Project/Area Number 24550108
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Analytical chemistry
Research InstitutionMeisei University

Principal Investigator

TASHIRO Mitsuru  明星大学, 理工学部, 教授 (40315750)

Co-Investigator(Kenkyū-buntansha) YOSHIMURA Etsuro  東京大学, 大学院農学生命科学研究科, 教授 (10130303)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords分子間相互作用 / タンパク質 / リガンド / 特異的検出 / 核磁気共鳴法 / 核磁気共鳴 / レセプター / 選択的検出 / 標的タンパク質 / 結合部位
Outline of Final Research Achievements

In acquiring NMR spectra, it is easier to use samples containing 100% D2O on the point of setting parameters. However, loss of valuable samples is inevitable due to the solvent exchange process. In the present research, the novel method, which is suitable to detect ligand binding to a protein receptor in solution containing 95% H2O+5% D2O, was developed. The pulse scheme, which can effectively attenuate huge water signal, was incorporated in STD and Water-LOGSY experiments. An advantage of the present method is the sensitive detection of the binding ligand in acquiring 1D spectra without using expensive isotopes such as 13C and/or 15N.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (22 results)

All 2015 2014 2013 2012

All Journal Article (15 results) (of which Peer Reviewed: 15 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (7 results)

  • [Journal Article] Structural basis for action of the external chaperone for a propeptide-deficient serine protease from Aeromonas sobria2015

    • Author(s)
      Hidetomo Kobayashi, Toru Yoshida, Takuya Miyakawa, Mitsuru Tashiro, Keinosuke Okamoto, Hiroyasu Yamanaka, Masaru Tanokura and Hideaki Tsuge
    • Journal Title

      J. Biol. Chem.

      Volume: 290 Pages: 11130-11143

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Achievement of 1H-19F heteronuclear experiments using the conventional spectrometer with a shared single high band amplifier2015

    • Author(s)
      Chiseko Sakuma, Jun-ichi Kurita, Kazuo Furihata and Mitsuru Tashiro
    • Journal Title

      Magn. Reson. Chem.

      Volume: 53 Issue: 5 Pages: 327-329

    • DOI

      10.1002/mrc.4210

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Analysis of weak affinity of beta-D-fructofuranosyl-(2↔1)-2-acetamido-2-deoxy-alpha- D-glucopyranoside for yeast beta-fructofuranosidase using NMR spectroscopy2014

    • Author(s)
      Chiseko Sakuma, Kazuo Furihata, Toshiyuki Nishio, Takuya Miyakawa, Masaru Tanokura and Mitsuru Tashiro
    • Journal Title

      J. Carbohyd. Chem.

      Volume: 33 Issue: 9 Pages: 498-505

    • DOI

      10.1080/07328303.2014.964407

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] BASHD-J-resolved-HMBC, an efficient method for measuring proton-proton and heteronuclear long range coupling constants2014

    • Author(s)
      Kazuo Furihata and Mitsuru Tashiro
    • Journal Title

      Magn. Reson. Chem.

      Volume: 52 Issue: 1-2 Pages: 27-31

    • DOI

      10.1002/mrc.4032

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Crystal Structure of 1,3,4,5-Tetra-O-acetyl-α-D-tagatopyranose2014

    • Author(s)
      Daisuke Miura, Takashi Fujimoto, Mitsuru Tashiro and Tomoya Machinami
    • Journal Title

      X-ray Structure Analysis Online

      Volume: 30 Pages: 3-4

    • NAID

      130004544324

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enzymatic synthesis of novel oligosaccharides from N-acetylsucrosamine using -fructofuranosidase from Aspergillus oryzae2013

    • Author(s)
      Toshiyuki Nishio, Mai Juami, Toru Wada, Yuta Sugimoto, Hiroki Senou, Wataru Komori, Chiseko Sakuma, Takako Hirano, Wataru Hakamata and Mitsuru Tashiro
    • Journal Title

      Carbohydrate Research

      Volume: 382 Pages: 108-112

    • DOI

      10.1016/j.carres.2013.10.006

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Crystal Structure of <i>&beta;</i>-<font size=-1>D</font>-Fructofuranosyl 4-chloro-4-deoxy-<i>&alpha;</i>-<font size=-1>D</font>-galactopyranoside2013

    • Author(s)
      Takashi Fujimoto, Daisuke Miura, Akari Iwamoto, Tomoya Machinami and Mitsuru Tashiro
    • Journal Title

      X-ray Structure Analysis Online

      Volume: 29 Issue: 0 Pages: 43-44

    • DOI

      10.2116/xraystruct.29.43

    • NAID

      130004544319

    • ISSN
      1883-3578
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Complex formation of 1,6-anhydro-&#61538;-maltose and sodium ions using single-crystal X-ray crystallography and NMR spectroscopy2013

    • Author(s)
      Takashi Fujimoto, Daisuke Miura, Takayuki Kato, Osamu Kamo, Yuichi Shimoikeda, Tomoya Machinami and Mitsuru Tashiro
    • Journal Title

      American Journal of Analytical Chemistry

      Volume: 4 Issue: 08 Pages: 361-367

    • DOI

      10.4236/ajac.2013.48045

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dopamine cannot promote oligomerization of unoxidized α-synuclein2013

    • Author(s)
      Sakurako Shimotakahara, Mika Matsui, Chiseko Sakuma, Teruaki Takahashi, Takashi Fujimoto, Kazuo Furihata, Masaki Kojima, Shohei Seino, Tomoya Machinami, Yoichi Shibusawa, Kenji Uéda and Mitsuru Tashiro
    • Journal Title

      J. Biophy. Chem.

      Volume: 4 Issue: 03 Pages: 110-114

    • DOI

      10.4236/jbpc.2013.43015

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] BASHD-J-resolved-COSY: a new method for measuring proton-proton spin coupling constants of multiplet signals2012

    • Author(s)
      K. Furihata and M. Tashiro
    • Journal Title

      Magn. Reson. Chem.

      Volume: 50 Issue: 11 Pages: 713-716

    • DOI

      10.1002/mrc.3866

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Selective COSY-J-resolved-HMBC, a new method for improving sensitivity of cross peaks of methine proton signals attached to a methyl group2012

    • Author(s)
      K. Furihata and M. Tashiro
    • Journal Title

      Magn. Reson. Chem.

      Volume: 50 Issue: 6 Pages: 409-414

    • DOI

      10.1002/mrc.3816

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Demonstration of three dopamine molecules bound to alopha-synuclein: Implication of oligomerization at the initial stage2012

    • Author(s)
      S Shimotakahara, Y Shiroyama, T Fujimoto, M Akai, T Onoue, H Seki, S Kado, T Machinami, Y Shibusawa, K Ueda, M Tashiro
    • Journal Title

      J. Biophysical Chem.

      Volume: 3 Issue: 02 Pages: 149-155

    • DOI

      10.4236/jbpc.2012.32017

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of methanol and acetonitrile eluents for the quantitation of chelators specific to soft-metal ions by HPLC2012

    • Author(s)
      S. Ogawa and E. Yoshimura
    • Journal Title

      J. Chromatogr. B

      Volume: 909 Pages: 34-36

    • DOI

      10.1016/j.jchromb.2012.10.005

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Substrate inhibition competes with halide inhibition in polyphenol oxidase2012

    • Author(s)
      G.G. Lim, Y. Imura and E. Yoshimura
    • Journal Title

      Protein J.

      Volume: 31 Issue: 7 Pages: 609-614

    • DOI

      10.1007/s10930-012-9442-5

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Micro Total Bioassay System for Oral Drugs: Evaluation of Gastrointestinal Degradation, Intestinal Absorption, Hepatic Metabolism, and Bioactivity2012

    • Author(s)
      Yuki IMURA, Etsuro YOSHIMURA, Kiichi SATO
    • Journal Title

      Analytical Sciences

      Volume: 28 Issue: 3 Pages: 197-197

    • DOI

      10.2116/analsci.28.197

    • NAID

      130004442113

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Achievement of 1H-19F heteronuclear experiments using the conventional spectrometer with a shared single high band amplifier2015

    • Author(s)
      Chiseko Sakuma, Jun-ichi Kurita, Kazuo Furihata, Mitsuru Tashiro
    • Organizer
      第56回 Experimental NMR Conference
    • Place of Presentation
      アシロマ、アメリカ
    • Year and Date
      2015-04-19 – 2015-04-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Observation of oligomerization process of &#61537;-synuclein in association with dopamine using NMR spectroscopy2014

    • Author(s)
      Kazuo Furihata, Chiseko Sakuma and Mitsuru Tashiro
    • Organizer
      55th Experimental NMR Conference
    • Place of Presentation
      Boston, MA, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] スクロースのクロロデオキシ誘導体のコンフォーメーション(変化)と甘味度増大との相関性2014

    • Author(s)
      岩本 明香里・三浦 大介・藤本 崇・町並 智也・田代 充
    • Organizer
      日本化学会第94春季年回(2014)
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
  • [Presentation] BASHD-J-Resolved-HMBC: A New Method for Measuring Heteronuclear Long Range Coupling Constants and Proton-Proton Coupling Constants of Multiplet Signals2013

    • Author(s)
      Kazuo Furihata and Mitsuru Tashiro
    • Organizer
      54th Experimental NMR Conference
    • Place of Presentation
      Pacific Grove, CA, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] BASHD-J-Resolved-COSY: A New Method for Measuring Proton-Proton Spin Coupling Constants of Multiplet Signals2012

    • Author(s)
      Kazuo Furihata and Mitsuru Tashiro
    • Organizer
      53rd Experimental NMR Conference
    • Place of Presentation
      Miami, Florida, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] HPLCポストカラム法を利用したカドミウムキレーターの高感度分析システムの開発とウマ血漿中グルタチオン分析への応用2012

    • Author(s)
      陳 嘉上・井村祐己・小川進也・吉村悦郎
    • Organizer
      第72回分析化学討論会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 蛍光物質Calcein Blueを用いたHPLCポストカラム法によるFe(III)キレーターの分析2012

    • Author(s)
      有賀智子・伊藤恭子・井村祐己・吉村悦郎
    • Organizer
      第72回分析化学討論会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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