Development of the specific detection methods to identify the binding epitope of ligand interacting with the target protein
| Project/Area Number |
24550108
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Meisei University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIMURA Etsuro 東京大学, 大学院農学生命科学研究科, 教授 (10130303)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 分子間相互作用 / タンパク質 / リガンド / 特異的検出 / 核磁気共鳴法 / 核磁気共鳴 / レセプター / 選択的検出 / 標的タンパク質 / 結合部位 |
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| Outline of Final Research Achievements |
In acquiring NMR spectra, it is easier to use samples containing 100% D2O on the point of setting parameters. However, loss of valuable samples is inevitable due to the solvent exchange process. In the present research, the novel method, which is suitable to detect ligand binding to a protein receptor in solution containing 95% H2O+5% D2O, was developed. The pulse scheme, which can effectively attenuate huge water signal, was incorporated in STD and Water-LOGSY experiments. An advantage of the present method is the sensitive detection of the binding ligand in acquiring 1D spectra without using expensive isotopes such as 13C and/or 15N.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Dopamine cannot promote oligomerization of unoxidized α-synuclein2013
Author(s)
Sakurako Shimotakahara, Mika Matsui, Chiseko Sakuma, Teruaki Takahashi, Takashi Fujimoto, Kazuo Furihata, Masaki Kojima, Shohei Seino, Tomoya Machinami, Yoichi Shibusawa, Kenji Uéda and Mitsuru Tashiro
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Journal Title
J. Biophy. Chem.
Volume: 4
Issue: 03
Pages: 110-114
DOI
Related Report
Peer Reviewed
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[Journal Article] Demonstration of three dopamine molecules bound to alopha-synuclein: Implication of oligomerization at the initial stage2012
Author(s)
S Shimotakahara, Y Shiroyama, T Fujimoto, M Akai, T Onoue, H Seki, S Kado, T Machinami, Y Shibusawa, K Ueda, M Tashiro
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Journal Title
J. Biophysical Chem.
Volume: 3
Issue: 02
Pages: 149-155
DOI
Related Report
Peer Reviewed
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