| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
In acquiring NMR spectra, it is easier to use samples containing 100% D2O on the point of setting parameters. However, loss of valuable samples is inevitable due to the solvent exchange process. In the present research, the novel method, which is suitable to detect ligand binding to a protein receptor in solution containing 95% H2O+5% D2O, was developed. The pulse scheme, which can effectively attenuate huge water signal, was incorporated in STD and Water-LOGSY experiments. An advantage of the present method is the sensitive detection of the binding ligand in acquiring 1D spectra without using expensive isotopes such as 13C and/or 15N.
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