| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In this study, to present the appropriate application of the size-exclusion chromatography combined with small-angle X-ray scattering technique, we will evaluate this measurement system by characterization of biological macromolecules, such as a large glycoprotein proteoglycan. This method will be useful for the estimation of the size (the radius of gyration value), zero-angle scattering intensity (molecular weight) and structural properties (conformations or persistence lengths) of separated biological macromolecules with dimensions of 1-10 nm in solution. The present results show that the combination of the small-angle X-ray and light scattering methods will be a powerful tool for characterization of large biopolymers in solution. Since the potential of this technique depends on the performance of the X-ray scattering instrument, further development of the measurement system will be important for future food and biological sciences.
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