Development of single nanoparticle measurement system for analysis of uptake behavior of nanoparticles in biological cells
| Project/Area Number |
24550112
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
INAGAKI KAZUMI 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究室長 (50356490)
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| Co-Investigator(Kenkyū-buntansha) |
TAKATSU Akiko 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究科長 (10357361)
FUJII Shin-ichirou 独立行政法人産業技術総合研究所, 計測標準研究部門, 主任研究員 (10415739)
MIYASHITA Shin-ichi 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究員 (60614766)
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| Co-Investigator(Renkei-kenkyūsha) |
KATO Haruhisa 独立行政法人産業技術総合研究所, 計測標準研究部門, 主任研究員 (10462839)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | ナノ粒子 / 細胞 / 質量分析 |
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| Outline of Final Research Achievements |
A single nanoparticle measurement system using ICP-MS was developed for analysis of uptake and dissolution behavior of nanoparticles in biological cells. In this study, a high speed detection system that can measure with time resolution less than 10 us was built in ICP-MS instrument, and an effective data processing scheme was established. Furthermore, direct cell introduction system was modified for extending a capability for introducing a large cells. For checking the performance of the system, the system developed was applied for evaluating uptake and dissolution behavior of nanoparticles in microalgal cells.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Separation and Quantification of RNA Molecules Using Size-Exclusion Chromatography Hyphenated with Inductively Coupled Plasma-Mass Spectrometry2014
Author(s)
Fujii,S., Inagaki, K., Miyashita, S., Groombridge, A. S., Nagasawa, K., Chiba, K., Takatsu, A.
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Journal Title
ELECTROPHORESIS
Volume: 35
Issue: 9
Pages: 1315-1318
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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