| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Outline of Final Research Achievements |
This study was carried out to construct effective synthesis methods for disease-related carbohydrates, leading to investigation of their structure-activity relationships. Particularly, tumor-associated type II sulfated carbohydrate antigens were the major synthesis targets in this study. This type of antigen has L-fucose branches, consisting of complex sulfated Lewis X units in a molecular chain. We first established a facile and rapid synthesis method for a sulfated Lewis X unit. We further found the novel methodology to obtain sulfated type II carbohydrate antigens containing L-fucose branches by the catalysis of keratanase II, which can transfer the sulfated Lewis X oxazoline derivative to the other type II sulfated chain. These important findings opened up the way to the synthesis of complex type II sulfated carbohydrate antigens for the first time.
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