Cell surface display of beta-peptide hydrolase and its application for beta-poly(Asp) synthesis
| Project/Area Number |
24550180
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental chemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
HIRAISHI TOMOHIRO 独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (20321804)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | βペプチド / ポリアスパラギン酸 / 分解酵素 / 細胞表層工学 / 酵素合成 / 進化工学 / バイオベースポリマー |
|---|
| Outline of Final Research Achievements |
The aim of this study is to develop the effective beta-poly(Asp) (beta-PAA) synthesis system by using whole-cell biocatalyst expressing PahZ1. The present results provided the detailed information about the substrate recognition of the enzyme. On the basis of this finding, we conducted the directed evolution of PahZ1 for the improvement of its function.
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Report
(4 results)
Research Products
(24 results)
