| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Site directed mutagenesis of cytochrome c3 from Desulfovibrio vulgaris was carried out for the addition of enzymatic activity to the electron transfer protein. Cytochrome c3 was tetraheme electron transfer protein and our previous study reveals the role of each heme. one of the heme plays an important role in electron donation to physiological electron acceptor, hydrogenase. So in this study, heme coordination sphere of the heme was changed by the site direct mutagenesis. Obtained cytochrome c3 can carried out both enzymatic activity and electron transfer activity.
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