Site direct mutagenesis of multiheme protein
| Project/Area Number |
24550183
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
KAMACHI Toshiaki 東京工業大学, 生命理工学研究科, 准教授 (30272694)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 電子伝達タンパク質 / シトクロムc3 / 光水素発生 / ヒドロゲナーゼ / ペルオキシダーゼ / 光機能化 |
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| Outline of Final Research Achievements |
Site directed mutagenesis of cytochrome c3 from Desulfovibrio vulgaris was carried out for the addition of enzymatic activity to the electron transfer protein. Cytochrome c3 was tetraheme electron transfer protein and our previous study reveals the role of each heme. one of the heme plays an important role in electron donation to physiological electron acceptor, hydrogenase. So in this study, heme coordination sphere of the heme was changed by the site direct mutagenesis. Obtained cytochrome c3 can carried out both enzymatic activity and electron transfer activity.
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Report
(4 results)
Research Products
(17 results)
