| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Outline of Final Research Achievements |
The final goal of this study is development of a high-throughput method for chemical synthesis of the genomic DNAs of mycoplasma and cyanobacteria. In this research, ‘improvement of chain elongation efficiency’, ‘simplification of purification of oligonucleotides’, and ‘development of a flow-system for synthesis of long oligonucleotides’ was carried out. Specifically, l the 30-mer DNA oligomers was synthesized by using the condensation reaction on the activator-contained solid phase carrier and a capping reaction with diphenylphosphonate. We also prepared a filter-type porous glass carrier for the flow system of DNA synthesis and examined the reactivity on the support in detail.
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