| Project/Area Number |
24550186
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
YAMAYOSHI Asako 京都工芸繊維大学, 工芸科学研究科, 助教 (70380532)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Akira 京都工芸繊維大学, 工芸科学研究科, 教授 (60210001)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | microRNA / アンチセンス核酸 / ペプチド / RISC / 核酸医薬 |
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| Outline of Final Research Achievements |
MicroRNAs are non-coding RNAs that induce post-transcriptional gene silencing of the target genes and regulate a wide range of biological processes including cancers. Therefore microRNAs are considered as attractive therapeutic targets for cancer therapy. MicroRNAs do not act alone, but exhibit the functions by forming RNA-induced silencing complex (RISC). Among the proteins of RISC components, Argonaute protein family has a highly conserved motif called PIWI-box that interacts with 5’-end of microRNAs. The interaction between PIWI-box and 5’-end of miRNA is an essential factor for holding microRNA in Argonaute. We designed peptides as an antagonist against PIWI-box, and then introduced these peptides to oligonucleotides targeting microRNAs. In this study, we evaluate the regulatory effects of peptide-conjugated oligonucleotides on RISC activity.
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