| Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
The charge transfer in i-motif and G-quadruplex DNA, which have a tetraplex structure, has been investigated by various time-resolved spectroscopies. The result showed that i-motif and G-quadruplex DNA can act as a good electron carrier or hole captor in DNA-based electronic devices.
Meanwhile, the RecA@ssDNA filament, which is formed by the interaction between RecA protein and G-rich sequence, was dissociated by the addition of K+ ions and the dissociated G-rich sequence was quickly folded to G-quadruplex structure. The conformation of G-quadruplex is converged to the specific G-quadruplex with one double-chain-reversal loop upon association of RecA protein. Furthermore, I found that the folding reaction of G-rich sequence (37htel) may undergo with two-state mechanism without any detectable intermediate and that the global structureis still occurring even after the formation of a secondary structure.
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