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Conformational dynamics of non-B DNA in vivo

Research Project

Project/Area Number 24550188
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Chemistry related to living body
Research InstitutionOsaka University

Principal Investigator

CHOI Jungkweon  大阪大学, 産業科学研究所, 助教 (00574328)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
KeywordsNon-B DNA / i-motif / G-quadruplex / Charge transfer / Folding / RecA protein / FRET / i-motif DNA / Electron Transfer / G-quadruple / 電荷分離
Outline of Final Research Achievements

The charge transfer in i-motif and G-quadruplex DNA, which have a tetraplex structure, has been investigated by various time-resolved spectroscopies. The result showed that i-motif and G-quadruplex DNA can act as a good electron carrier or hole captor in DNA-based electronic devices.
Meanwhile, the RecA@ssDNA filament, which is formed by the interaction between RecA protein and G-rich sequence, was dissociated by the addition of K+ ions and the dissociated G-rich sequence was quickly folded to G-quadruplex structure. The conformation of G-quadruplex is converged to the specific G-quadruplex with one double-chain-reversal loop upon association of RecA protein. Furthermore, I found that the folding reaction of G-rich sequence (37htel) may undergo with two-state mechanism without any detectable intermediate and that the global structureis still occurring even after the formation of a secondary structure.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (18 results)

All 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (12 results) (of which Invited: 1 results)

  • [Journal Article] Dynamics in the heme geometry of myoglobin induced by the one-electron reduction2014

    • Author(s)
      Junggkweon Choi, Sachiko Tojo, Mamoru Fujitsuka, and Tetsuro Majima
    • Journal Title

      International Journal of Radiation Biology

      Volume: 90 Issue: 6 Pages: 7-7

    • DOI

      10.3109/09553002.2013.876115

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reversible Conformational Switching of i-motif DNA Studied by Fluorescence Spectroscopy2013

    • Author(s)
      J. Choi and T. Majima
    • Journal Title

      Photochem. Photobiol.

      Volume: 89 Issue: 3 Pages: 513-522

    • DOI

      10.1111/php.12042

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Interaction of G-quadruplex with RecA Protein Studied in Bulk Phase and at the Single-Molecule Level2013

    • Author(s)
      A. Tanaka, J. Choi, S. K. Kim and T. Majima
    • Journal Title

      J. Phys. Chem. B

      Volume: 117 Issue: 22 Pages: 6711-6717

    • DOI

      10.1021/jp4036277

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Efficient Electron Transfer in i-motif DNA with a Tetraplex Structure2013

    • Author(s)
      J. Choi, A Tanaka, D. W. Cho, M. Fujitsuka, and T. Majima
    • Journal Title

      Angew. Chem. Int. Ed.

      Volume: 52 Issue: 49 Pages: 12937-12941

    • DOI

      10.1002/anie.201306017

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hole-Trapping of G-Quartets in a G-Quadruplex2013

    • Author(s)
      J. Choi, M. Fujitsuka, T. Majima
    • Journal Title

      Angew. Chem. Int. Ed

      Volume: 52 Issue: 4 Pages: 1134-1138

    • DOI

      10.1002/anie.201208149

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Folding Dynamics of Cytochrome c Using Pulse Radiolysis2012

    • Author(s)
      J. Choi, M. Fujitsuka, T. Majima
    • Journal Title

      J. Am. Chem. Soc

      Volume: 134 Issue: 32 Pages: 13430-13435

    • DOI

      10.1021/ja304904p

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] GGG配列を6つ有するヒトテロメア鎖のG四重鎖形成とその構造多様性2014

    • Author(s)
      Atsushi Tanaka, Jungkweon Choi* and Tetsuro Majima*
    • Organizer
      第36回日本光医学・光生物学会
    • Place of Presentation
      大阪大学医学部学友会館・銀杏会館
    • Year and Date
      2014-07-25 – 2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Charge Transfer in Non-B DNA with a Tetraplex Structure2014

    • Author(s)
      Jungkweon Choi, Atsushi Tanaka, Mamoru Fujitsuka and Tetsuro Majima
    • Organizer
      The 17th SANKEN International Symposium 2014
    • Place of Presentation
      大阪、大阪大学、Icho Kaikan
    • Related Report
      2013 Research-status Report
  • [Presentation] G-四重鎖のG-カルテットにおける正孔捕獲2013

    • Author(s)
      崔 正権、田中 敦志、Man Jae Park、藤塚 守、真嶋 哲朗
    • Organizer
      日本光医学・光生物学会
    • Place of Presentation
      静岡県浜松市、アクトシティ浜松コングレスセンター
    • Related Report
      2013 Research-status Report
  • [Presentation] Folding dynamics of cytochrome c investigated by pulse radiolysis2013

    • Author(s)
      Jungkweon Choi, Mamoru Fujitsuka, Sachiko Tojo, Tetsuro Majima
    • Organizer
      The 16th SANKEN International Symposium 2013
    • Place of Presentation
      大阪、大阪大学、産業科学研究所
    • Related Report
      2012 Research-status Report
  • [Presentation] G-四重鎖のG-カルテットにおける正孔捕獲2013

    • Author(s)
      崔正権、田中敦志、藤塚守、真嶋哲朗
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      滋賀県草津市、立命館大学びわこ・くさつキャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] シトクロムcの折り畳み過程の研究2012

    • Author(s)
      崔 正権、藤塚 守、藤乗幸子、真嶋哲朗
    • Organizer
      第34回日本光医学・光生物学会
    • Place of Presentation
      神戸商工会議所と神戸国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] シトクロムcの折り畳み過程の研究2012

    • Author(s)
      崔 正権、藤塚 守、藤乗幸子、真嶋哲朗
    • Organizer
      第17回日本光生物学協会年会
    • Place of Presentation
      大阪、大阪大学、産業科学研究所
    • Related Report
      2012 Research-status Report
  • [Presentation] pH変化によるi-motif DNAの構造変化のダイナミクス2012

    • Author(s)
      金 水縁、崔 正権、藤塚 守、藤乗幸子、真嶋哲朗
    • Organizer
      第17回日本光生物学協会年会
    • Place of Presentation
      大阪、大阪大学、産業科学研究所
    • Related Report
      2012 Research-status Report
  • [Presentation] 単一分子レベルでのポリアデニンの自己集合に関する研究2012

    • Author(s)
      崔 正権、金 水縁、真嶋哲朗
    • Organizer
      光化学討論会
    • Place of Presentation
      東京、東京工業大学 大岡山キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] Folding/Unfolding Dynamics of Cytochrome c2012

    • Author(s)
      Jungkweon Choi, Tetsuro Majima
    • Organizer
      KCS 110th National Meeting
    • Place of Presentation
      BEXCO, Busan, Korea
    • Related Report
      2012 Research-status Report
  • [Presentation] Electron Transfer-triggered Folding Dynamics of Cytochrome c2012

    • Author(s)
      Jungkweon Choi, Mamoru Fujitsuka, Sachiko Tojo, and Tetsuro Majima
    • Organizer
      7th Asian Photochemistry Conference
    • Place of Presentation
      大阪、大阪大学、Icho Kaikan
    • Related Report
      2012 Research-status Report
  • [Presentation] Dynamics of non-B DNA: G-quadruple, i-motif and A-motif

    • Author(s)
      Jungkweon Choi
    • Organizer
      日本化学会アジア国際シンポジウム光化学Division
    • Place of Presentation
      滋賀県草津市、立命館大学びわこ・くさつキャンパス
    • Related Report
      2012 Research-status Report
    • Invited

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Published: 2013-05-31   Modified: 2021-04-07  

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