Development of the artificial enzyme using visible light with vitamin B12 derivatives and human serum albumin.

• Project/Area Number: 24550191
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Kyushu University
• Principal Investigator: MASUKO Takahiro 九州大学, 工学(系)研究科(研究院), 技術専門職員 (40457445)
• Co-Investigator(Renkei-kenkyūsha): HISAEDA Yoshio 九州大学, 大学院工学研究院, 教授 (70150498)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: ビタミンB12 / 触媒反応 / 金属酵素 / 光触媒 / バイオインスパイアード / 光増感剤

Outline of Final Research Achievements

The organic halide used for a pesticide is left to soil and the groundwater at long time. These continue affecting the creature until it is decomposed. On the other hand, it is known that the dehalogenase of the naturally occurring vitamin B12-dependent enzyme does de-chlorination reaction of the tetrachloroethylene. In this study, I succeeded in the development of the artificial enzyme using visible light with vitamin B12 derivatives and human serum albumin in water catalytically.

Research Products

• [Journal Article] Dechlorination of DDT Catalyzed by Visible-Light-Driven System Composed of Vitamin B_<12> Derivative and Rhodamine B (2013)
  • Author(s): K. Tahara, K. Mikuriya, T. Masuko, J. Kikuchi, Y. Hisaeda
  • Journal Title: J. Porphyr. Phthalocya Volume: Vol.17, No.1-2 Issue: 01n02 Pages: 135-141
  • DOI: 10.1142/s1088424612501398
  • Peer Reviewed
• [Journal Article] Synthesis, Characterization, Co-S Bond of a Vitamin B12 Model Complex Having Pentafluorophenylthiolate as an Axial Ligand (2013)
  • Author(s): Tahara, K.; Matsuzaki, A.; Masuko, T.; Kikuchu, J.; Hisaeda, Y.
  • Journal Title: Dalton Trans. Volume: 42 Issue: 18 Pages: 6410-6416
  • DOI: 10.1039/c3dt00042g
  • Peer Reviewed
• [Journal Article] Bioinspired catalytic reactions with vitamin B12 derivative and photosensitizers (2013)
  • Author(s): Y. Hisaeda, K. Tahara, H. Shimakoshi, T. Masuko
  • Journal Title: Pure and Applied Chemistry Volume: 85 Issue: 7 Pages: 1415-1426
  • DOI: 10.1351/pac-con-12-10-05
  • Peer Reviewed
• [Presentation] Inhibitory Effect of a Novel Porphobilinogen Analog on the Synthesis of a Heme Precursor by Hydroxymethylbilane Synthase (2015)
  • Author(s): Hideaki SATO
  • Organizer: 19th International Conference on Cytochrome(ICCP450 Tokyo 2015）
  • Place of Presentation: 東京都渋谷区・国立オリンピック記念青少年総合センター
  • Year and Date: 2015-06-12 – 2015-06-15
• [Presentation] ヒドロキシメチルブランシンターゼに対する 2-ヨードポルホビリノーゲンの阻害効果と複合体の立体構造の解析 (2015)
  • Author(s): 佐藤秀明
  • Organizer: 日本生化学九州支部例会
  • Place of Presentation: 福岡県福岡市東区・九州大学箱崎キャンパス
  • Year and Date: 2015-05-16 – 2015-05-17
• [Presentation] 新規ポルホビリノーゲン誘導体のヒドロキシメチルビランシンターゼに対する阻害剤としての効果 (2015)
  • Author(s): 佐藤秀明
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 千葉県船橋市・日本大学理工学部船橋キャンパス
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] B12酵素に学ぶバイオインスパイアード触媒の創製と触媒機能 (2015)
  • Author(s): 久枝良雄
  • Organizer: 分子研研究会
  • Place of Presentation: 愛知県岡崎市・岡崎コンファレンスホール
  • Year and Date: 2015-01-06 – 2015-01-07
• [Presentation] 人工酵素 バイオインスパイアード触媒 (2014)
  • Author(s): 久枝良雄
  • Organizer: CERI公開講座「先端材料化学&#12316;設計、構造・物性から機能化まで&#12316;」
  • Place of Presentation: 福岡市産学連携交流センター
  • Year and Date: 2014-12-06
• [Presentation] バイオインスパイアード錯体化学＜ピロール系金属錯体の化学＞ (2014)
  • Author(s): 久枝良雄
  • Organizer: 第3回錯体化学若手の会 九州・沖縄支部勉強会
  • Place of Presentation: 九州大学箱崎キャンパス
  • Year and Date: 2014-11-29
  • Invited
• [Presentation] Bioinspired Catalysts with Vitamin B12 Derivative and Photosensitizers (2013)
  • Author(s): Hisaeda, Y.
  • Organizer: 20th International SPACC Symposium
  • Place of Presentation: Changchun, China
• [Presentation] Synthesis and Photosensitizing Property of Porphycene with Ionic Liquid Tag (2013)
  • Author(s): Hisaeda, Y.
  • Organizer: 20th International SPACC Symposium
  • Place of Presentation: Changchun, China
• [Presentation] Synthesis and Characterization of a Hydrophobic Vitamin B12 Model Complex Having a Co-S Bond (2013)
  • Author(s): Tahara, K.
  • Organizer: Eighth International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP13)
  • Place of Presentation: 立命館大学
• [Presentation] Bioinspired Catalysts with Cobalt Complexes and Photosensitizers: Lessons from Vitamin B12-dependent Enzymes (2013)
  • Author(s): Hisaeda, Y.
  • Organizer: 6th International Conference on Green and Sustainable Chemistry (GSC-6)
  • Place of Presentation: The University of Nottingham
• [Presentation] Bioinspired Catalysts with Photosensitizers (2012)
  • Author(s): Hisaeda, Y.
  • Organizer: The International Symposium on Preparative Chemistry of Advanced Materials-2012
  • Place of Presentation: Liaoning University, Shenyang・China
  • Invited
• [Presentation] ビタミンB12誘導体によるチオールの触媒的酸化反応 (2012)
  • Author(s): 松崎彩夏
  • Organizer: 第6回 バイオ関連化学シンポジウム
  • Place of Presentation: 北海道大学
• [Presentation] Bioinspired Catalysts with Vitamin B12 Derivative and Photosensitizer
  • Author(s): Yoshio Hisaeda
  • Organizer: 9th JCSMCC（第9回日中クラスター会議）
  • Place of Presentation: 福岡
• [Remarks] 久枝研究室 HISAEDA's Laboratory
  • URL: http://www.chem.kyushu-u.ac.jp/~hisaeda/