Development of a new efficient methodology to isolate trace amounts of proteins in cells
| Project/Area Number |
24550195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KISHIMOTO Toshihiko 東邦大学, 理学部, 教授 (90339200)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 生体機能関連化学 / タンパク質の単離精製 / 光分解 / クリックケミストリー |
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| Outline of Final Research Achievements |
In this research project, we have been trying to develop a new efficient methodology to isolate trace amounts of proteins in cells. The surface of three kinds of beads were modified by compounds having photolabile groups and afforded to react with soluble molecules by Click Chemistry. Capture and photorelease of model proteins on modified beads were also examined.
Modification of the surface of beads was conducted by conventional solid phase peptide synthesis. Although Click Chemistry on the beads was confirmed, copper ion, which is catalyst for Click Chemistry, might promote nonspecific absorption of proteins upon beads surface. Model proteins bound on beads surface were released upon irradiation, but quantitative evaluation will be needed. Identification of photoproducts using model compounds was also underway.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Katanin p60 contributes to microtubule instability around the midbody and facilitates cytokinesis in rat cells2013
Author(s)
M. Matsuo, T. Shimodaira, T. Kasama, Y. Hata, A. Echigo, M. Okabe, K. Arai, Y. Makino, S. Niwa, H. Saya, T. Kishimoto
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Journal Title
PLoS ONE
Volume: 8
Issue: 11
Pages: e80392-e80392
DOI
Related Report
Peer Reviewed
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[Presentation] 疑似進化による新規的表現型の獲得
Author(s)
應 ベイウェン, 本田 朋也, 吉田 真理, 岸本 利彦, 津留 三良, 四方 哲也
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡国際会議場（福岡県）
Related Report
