| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Outline of Final Research Achievements |
A series of fluorescent probes for hydrogen peroxide have been developed that have a mononuclear nonheme iron complex as a reaction site toward hydrogen peroxide. They are all non-fluorescent, but once reacting with low concentrations of hydrogen peroxide inside cells, they release green- or red-emitting fluorescent dyes such as resorufin and fluorescein. We have succeeded in real-time imaging of hydrogen peroxide that was produced as a messenger inside A431 cells upon stimulation of epidermal growth factors.
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