• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of Rapid and Sensitive Fluorescent Probes for Hydrogen Peroxide Bio-imaging

Research Project

Project/Area Number 24550197
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Chemistry related to living body
Research InstitutionDoshisha University

Principal Investigator

HITOMI Yutaka  同志社大学, 理工学部, 教授 (20335186)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywordsイメージング / 蛍光 / 過酸化水素 / 検出 / 細胞 / 錯体 / シグナル / 鉄錯体 / 蛍光プローブ
Outline of Final Research Achievements

A series of fluorescent probes for hydrogen peroxide have been developed that have a mononuclear nonheme iron complex as a reaction site toward hydrogen peroxide. They are all non-fluorescent, but once reacting with low concentrations of hydrogen peroxide inside cells, they release green- or red-emitting fluorescent dyes such as resorufin and fluorescein. We have succeeded in real-time imaging of hydrogen peroxide that was produced as a messenger inside A431 cells upon stimulation of epidermal growth factors.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (22 results)

All 2015 2014 2013 2012

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) (of which Invited: 4 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Facile preparation of stable aqueous titania sols for fabrication of highly active TiO2 photocatalyst films2015

    • Author(s)
      S. Okunaka, *H. Tokudome, Y. Hitomi, *R. Abe
    • Journal Title

      J. Mater. Chem. A

      Volume: 3 Issue: 4 Pages: 1688-1695

    • DOI

      10.1039/c4ta04680c

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis, Stability and Reactivity of the First Mononuclear Nonheme Oxoiron(IV) Species with Monoamido Ligation: A Putative Reactive Species Generated from Iron-Bleomycin2014

    • Author(s)
      *Y. Hitomi, K. Arakawa, M. Kodera
    • Journal Title

      Chem. Commun.

      Volume: 50 Issue: 56 Pages: 7485-7487

    • DOI

      10.1039/c4cc01409j

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gold Nanoparticles Coated with Manganese-Porphyrin that Effectively Shorten the Longitudinal Relaxation Time of Water Molecules Depending on the Particle Size2014

    • Author(s)
      *Y. Hitomi, K. Aoki, R. Miyachi, J. Ohyama, M. Kodera, T. Tanaka, F. Sugihara
    • Journal Title

      Chem. Lett.

      Volume: 43 Issue: 12 Pages: 1901-1903

    • DOI

      10.1246/cl.140812

    • NAID

      130004687290

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Green-Emitting Iron Complex-Based Fluorescent Probes for Intracellular Hydrogen Peroxide Imaging2014

    • Author(s)
      Yutaka Hitomi, Toshiyuki Takeyasu, Masahito Kodera
    • Journal Title

      Bulletin of the Chemical Society of Japan

      Volume: (印刷中) Issue: 7 Pages: 819-824

    • DOI

      10.1246/bcsj.20140055

    • NAID

      130004059123

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Water Proton Relaxivity, Superoxide Dismutase-like Activity, and Cytotoxicity of a Manganese(III) Porphyrin Having Four Poly(ethylene glycol) Tails2014

    • Author(s)
      *Y. Hitomi, T. Ekawa, M. Kodera
    • Journal Title

      Chem. Lett.

      Volume: 43 Issue: 5 Pages: 732-734

    • DOI

      10.1246/cl.140029

    • NAID

      130004868139

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electronic Tuning of Nitric Oxide Release from Manganese Nitrosyl Complexes by Visible Light Irradiation: Enhancement of Nitric Oxide Release Efficiency by Nitro-Substituted Quinoline Ligand2014

    • Author(s)
      *Y. Hitomi, Y. Iwamoto, M. Kodera
    • Journal Title

      Dalton Trans.

      Volume: 43 Issue: 5 Pages: 2161-2167

    • DOI

      10.1039/c3dt51719e

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of Carboxylate Donors in O-O Bond Scission of Peroxodiiron(III) to High-Spin Oxodiiron(IV) with a New Carboxylate-Containing Dinucleating Ligand2014

    • Author(s)
      M. Kodera, T. Tsuji, T. Yasunaga, Y. Kawahara, T. Hirano, Y. Hitomi, T. Nomura, T. Ogura, Y. Kobayashi, Sajith P. K., Y. Shiota, and K. Yoshizawa
    • Journal Title

      Chem. Sci.

      Volume: 5 Issue: 6 Pages: 2282-2292

    • DOI

      10.1039/c3sc51541a

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Nucleophilic Ring-opening of meso-Substituted 5-Oxaporphyrin by Oxygen, Nitrogen, Sulfur, and Carbon Nucleophiles2014

    • Author(s)
      K. Kakeya, M. Aozasa, *T. Mizutani, Y. Hitomi, M. Kodera
    • Journal Title

      J. Org. Chem.

      Volume: 79 Issue: 6 Pages: 2591-2600

    • DOI

      10.1021/jo5000412

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞内の過酸化水素を可視化するプローブ分子の開発2014

    • Author(s)
      武安俊幸、人見 穣、小寺政人
    • Journal Title

      同志社大学理工学研究報告

      Volume: 55 Pages: 1-9

    • NAID

      110009799484

    • Related Report
      2013 Research-status Report
  • [Presentation] Selective Oxidation by Mononuclear Oxoiron Complexes with Monoamido Ligation2014

    • Author(s)
      Y. Hitomi
    • Organizer
      錯体化学会第64回討論会(2014)シンポジウム
    • Place of Presentation
      東京都文京区
    • Year and Date
      2014-09-18 – 2014-09-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Metal Complex-Based Fluorescent Probes for Imaging Hydrogen Peroxide2014

    • Author(s)
      Y. Hitomi
    • Organizer
      41st International Conference on Coordination Chemistry (ICCC)
    • Place of Presentation
      Singapore
    • Year and Date
      2014-07-21 – 2014-07-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Alkane Hydroxylation by Oxoiron Species Supported by Pentadentate N5 Ligands with Monoamido Coordination2014

    • Author(s)
      Y. Hitomi
    • Organizer
      International Conference on Hydrogen Atom Transfer (iCHAT 2014)
    • Place of Presentation
      Italy
    • Year and Date
      2014-06-22 – 2014-06-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of Metal Complexes Working in Cells: Fluorescent Probes for Intracellular Hydrogen Peroxide2013

    • Author(s)
      人見 穣
    • Organizer
      錯体化学会第63回討論会
    • Place of Presentation
      琉球大学千原キャンパス
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 細胞内の過酸化水素の可視化を目指した金属錯体型蛍光プローブの開発2013

    • Author(s)
      武安 俊幸・人見 穣・小寺 政人
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 金属錯体型蛍光プローブを利用した生体内活性酸素種の検出2013

    • Author(s)
      武安 俊幸,人見  穣,小寺 政人
    • Organizer
      第66回日本酸化ストレス学会
    • Place of Presentation
      ウインク愛知
    • Related Report
      2013 Research-status Report
  • [Presentation] 金属錯体型H2O2蛍光プローブの開発(1)2013

    • Author(s)
      武安俊幸・人見 穣・小寺政人
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] 金属錯体型H2O2蛍光プローブの開発(2)2013

    • Author(s)
      高野弘都・武安俊幸・人見 穣・小寺政人
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] 金属錯体を利用した過酸化水素の蛍光検出2012

    • Author(s)
      武安 俊幸・人見 穣・小寺 政人
    • Organizer
      第6回バイオ関連化学シンポジウム
    • Place of Presentation
      北海道大学高等教育推進機構
    • Related Report
      2012 Research-status Report
  • [Presentation] 水中でペルオキシダーゼ活性を示す単核鉄(III)錯体の開発2012

    • Author(s)
      平松 和明・荒川 健吾・人見 穣・小寺 政人
    • Organizer
      錯体化学会 第62回討論会
    • Place of Presentation
      富山大学五福キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] 過酸化水素の高感度検出を目的とした金属錯体蛍光プローブの開発2012

    • Author(s)
      武安俊幸・人見 穣・小寺政人
    • Organizer
      第45回酸化反応討論会
    • Place of Presentation
      名古屋市立大学病院
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 複素環化合物,金属錯体および蛍光プローブ2013

    • Inventor(s)
      人見 穣、武安 俊幸
    • Industrial Property Rights Holder
      学校法人同志社大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-07-29
    • Related Report
      2013 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 複素環化合物,金属錯体および蛍光プローブ2012

    • Inventor(s)
      人見 穣、武安 俊幸
    • Industrial Property Rights Holder
      人見 穣、武安 俊幸
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-08-27
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi