| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Outline of Final Research Achievements |
DNA methylation is involved in the regulation of many epigenetic processes, including embryonic development and cellular differentiation. DNA methylation particularly appears at the 5’-position of cytosine residue in 5’-CpG-3’ sequence. In addition, erroneous DNA methylation may contribute to the etiopathogenesis of tumorigenesis and aging. The inheritance of DNA methylation is coupled to the DNA replication process, however, the mechanisms in detail involving hemimethylation is not completely clarified, in which only one of two complementary strands is methylated. The development of a new detection method of hemimethylation should contribute to coming epigenetics research.
In this study, an artificial protein/peptide has been developed that bind preferentially to a hemimethylated DNA rather than the full-methylated and unmethylated DNAs.
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