Recognition of metal-mediated base pairs by DNA polymerases
| Project/Area Number |
24550199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University of Pharmaceutical Sciences |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 金属錯体型塩基対 / DNAポリメラーゼ / プライマー伸長反応 / 銀(I)イオン / metal-mediated base pair / DNA polymerase / Ag(I) ion / primer extension / Ag(I)-mediated base pair |
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| Outline of Final Research Achievements |
In this project, first we studied the DNA polymerase-catalyzed primer extension in the presence of Ag(I) ions and unexpectedly found the formation of not the C-Ag(I)-C base pair but the C-Ag(I)-A base pair. Subsequently, we found the formation of the C-Ag(I)-T base pair by exhaustive screening of DNA polymerase-catalyzed mismatch base pair formation under the presence of various kinds of metal ions. By using very high specificity of the formation of the C-Ag(I)-T base pair and the previously reported T-Hg(II)-T base pair, we demonstrated that two kinds of metal ions, Ag(I) and Hg(II), highly selectively mediate the incorporation of thymidine 5’-triphosphate (dTTP) into the sites opposite cytosine and thymine in the template, respectively. In other words, the regulated incorporation of different metal ions into the programmed sites in duplex by DNA polymerase was successfully achieved.
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Report
(4 results)
Research Products
(14 results)
