Study on the controlling mechanism of fibrin gel formation by the unordered region of fibrinogen and N-linked saccharide chain
Project/Area Number |
24550245
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Polymer/Textile materials
|
Research Institution | Gunma University |
Principal Investigator |
KUBOTA Kenji 群馬大学, 大学院理工学府, 教授 (40153332)
|
Co-Investigator(Kenkyū-buntansha) |
NAMEKI Nobukazu 群馬大学, 大学院理工学府, 准教授 (80302959)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | ゲル / 生体高分子 / フィブリノゲン / Bβ鎖N末端領域 / ラテラル凝集 / Fragment-D / 表面プラズモン共鳴 / Bβ鎖N末端領域 / N結合糖鎖 / フィブリン重合 / NDSK / Fragment-D / SPR / フィブリンゲル / N結合糖鎖 |
Outline of Final Research Achievements |
The following schemes in the fibrin gel formation process were strongly suggested by the SPR measurements using recombinant (and mutated) fibrinogen fragment corresponding to the N-terminal region of fibrinogen Bβ chain: 66 amino acid residues in the N-terminal region of fibrinogen Bβpolypeptide chain (BβN) play an essential role in the fibrin gel formation through interacting with the distal D-regions of fibrinogen. The central factor in that interaction is the unordered region with high mobility around Bβ44R, especially Bβ44R contributes notably to the interaction. N-linked carbohydrate chain inhibitingly affects the interaction between BβN and D-region, thus suppresses the lateral aggregation of protofibrils.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Identification of residues required for stalled-ribosome rescue in the codon-independent release factor YaeJ.2014
Author(s)
Kogure, H., Handa, H., Nagata, M., Kanai, N., Guntert, P., Kubota, K., Nameki, N.
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Journal Title
Nucleic Acids Research
Volume: 42
Issue: 5
Pages: 3152-3163
DOI
Related Report
Peer Reviewed
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