Development of novel piezoelectric materials with optical absorber and its application to gene transfer by combination of laser induced shock wave and pulsed electric field
| Project/Area Number |
24560384
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Electronic materials/Electric materials
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| Research Institution | Kanazawa Institute of Technology |
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Principal Investigator |
AIZAWA Koji 金沢工業大学, 工学部, 教授 (40222450)
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| Co-Investigator(Kenkyū-buntansha) |
KOGI Mieko 金沢工業大学, バイオ・化学部, 教授 (50410288)
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| Co-Investigator(Renkei-kenkyūsha) |
TOKUNAGA Yoshiaki 金沢工業大学, 工学部, 教授 (00072174)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 圧電体 / レーザ / 衝撃波 / 電界 / 遺伝子導入 |
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| Outline of Final Research Achievements |
Laser induced shock wave (LISW) is used for gene transfer into cells. LISW is a shock wave because its propagation velocity is higher than the sound speed. A proposed device structure can generate high pulsed electric field due to piezoelectricity when LISW propagates in this structure. We consider that this pulsed electric field is also effective on gene transfection. In this study, we clarified the experimental conditions of pulsed electric field generation by LISW irradiation, and experimentally showed the shock wave formation in the confined structure using 0.05 mm-thick black rubber target even when the laser fluence is as low as 0.4 J/cm2. We also investigated the transfection efficiency of FITC dextran to several cell species when an LISW generated by thin black rubber target (0.07 mm in thickness) was irradiated to their cells, and clarified the experimental conditions in order to improve the transfection efficiency.
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Report
(4 results)
Research Products
(45 results)
