Quantitative detection of infectious Legionella spp. in water supply systems using their virulence factors as markers
| Project/Area Number |
24560663
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Azabu University (2013-2014)
Kyoto University (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 水系感染症微生物 / レジオネラ / 感染能 / 再増殖 / 残留塩素 |
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| Outline of Final Research Achievements |
The change of infectivity of Legionella pneumophila was examined using a co-culture system with a THP-1 cell line. The survival of L. pneumophila in THP-1 was drastically increased by L-cystein depletion and by exposure to low organic carbon condition. Also, the contamination with Legionella spp. in water supply systems with cisterns was surveyed. The cultivable Legionella spp. was detected in approximately 30% faucets with stagnant water, and the regrowth was observed in even the samples which cultivable Legionella spp. was undetectable at each sampling. These results suggest that VBNC Legionella reside in water supply systems.
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Report
(4 results)
Research Products
(8 results)
