Development of new aortic stent-graft materials by plasma technology that can control the surface morphology and the drug elution

• Project/Area Number: 24560860
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural/Functional materials
• Research Institution: Tokai University
• Principal Investigator: HASEBE Terumitsu 東海大学, 医学部, 教授 (20306799)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Tetsuya 慶應義塾大学, 理工学部, 教授 (10286635)
• Co-Investigator(Renkei-kenkyūsha): HOTTA Atsushi 慶應義塾大学, 理工学部, 教授 (30407142)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 新機能材料 / 非晶質炭素膜 / リン脂質ポリマー / マイクロパターニング / 薬剤徐放 / プラズマ技術 / 細胞接着制御 / ステントグラフト内 / エンドリーク / 炭素系薄膜 / プラズマ / 薬剤徐放部材 / 血管内皮細胞 / 線維芽細胞

Outline of Final Research Achievements

Endovascular aneurysm repair (EVAR) using a stent-graft is designed to help prevent an aneurysm from bursting. Endoleaks are characterized by persistent blood flow within the aneurysm sac following EVAR. In this study, to inhibit the endoleak inflow, we controlled the surface morphology and the drug elution of graft material by plasma technology, and we developed new materials for a graft fabric that enhanced cell adhesion and proliferation. As the specific results, we revealed that (1) the control of the surface morphology and the drug emission contributed to the proliferation of endothelial cells and that (2) micro-patterned hydrogenated amorphous carbon (a-C:H) coating on the polymer impregnated with basic fibroblast glows factor (bFGF) improved the endothelial cells growth rate.

Research Products

• [Journal Article] Balloon-occluded transarterial chemoembolization using a 1.8-French tip Coaxial microballoon catheter for hepatocellular carcinoma: Technical and safety considerations. (2015)
  • Author(s): Matsumoto T*, Endo J, Hashida K, Ichikawa H, Kojima S, Takashimizu S, Watanabe N, Yamagami T, Hasebe T* (* Matsumoto T and Hasebe T equally contributed to this work.)
  • Journal Title: Minim Invasive Ther Allied Technol Volume: 24 Pages: 94-100
  • Peer Reviewed
• [Journal Article] Preparation and characterization of 2-methacryloyloxyethyl phosphorylcholine polymer nanofibers prepared via electrospinning for biomedical materials (2014)
  • Author(s): Maeda, T., Hagiwara, K., Yoshida, S., Hasebe, T. & Hotta, A.
  • Journal Title: Journal of Applied Polymer Science Volume: 131 Issue: 14 Pages: 40606-40606
  • DOI: 10.1002/app.40606
  • Peer Reviewed
• [Journal Article] Effectiveness of combined use of imprint cytological and histological examination in CT-guided tissue-core biopsy. (2014)
  • Author(s): Yamagami T, Yoshimatsu R, Kajiwara K, Ishikawa M, Matsumoto T, Kakizawa H, Toyoda N, Hasebe T, Awai K
  • Journal Title: Eur Radiol Volume: 24 Pages: 1127-1134
  • Peer Reviewed
• [Journal Article] Basic Fibroblast Growth Factor as a Potential Stent Coating Material Inducing Endothelial Cell Proliferation (2014)
  • Author(s): Kitamura, N., Hasebe, T., Matsumoto, T, Hotta, A., Suzuki, T., Yamagami, T. & Terada, H.
  • Journal Title: Journal of Atherosclerosis and Thrombosis Volume: 21 Issue: 5 Pages: 477-485
  • DOI: 10.5551/jat.20404
  • NAID: 130004444732
  • ISSN: 1340-3478, 1880-3873
  • Peer Reviewed
• [Journal Article] Surface modification of polymers by plasma treatments for the enhancement of biocompatibility and controlled drug release (2013)
  • Author(s): Yoshida, S., Hagiwara, K., Hasebe, T. & Hotta, A.
  • Journal Title: Surface and Coating Technology Volume: - Pages: 99-107
  • DOI: 10.1016/j.surfcoat.2013.02.042
  • Peer Reviewed
• [Journal Article] Hydrophobicity and non-thrombogenicity of nanoscale dual rough surface coated with fluorine-incorporated diamond-like carbon films: Biomimetic surface for blood-contacting medical devices (2013)
  • Author(s): Hasebe, T., Nagashima, S., Kamijo, A., Moon, M.W., Kashiwagi, Y., Hotta, A., Lee, K.R., Takahashi, K., Yamagami, T. & Suzuki, T.
  • Journal Title: Diamond and Related Materials Volume: 38 Pages: 14-18
  • DOI: 10.1016/j.diamond.2013.06.001
  • Peer Reviewed
• [Journal Article] Ultrastructural characterization of surface-induced platelet activation on artificial materials by transmission electron microscopy (2013)
  • Author(s): Yoshimoto, Y., Hasebe, T., Takahashi, K., Amari, M., Nagashima, S., Kamijo, A., Hotta, A., Takahashi, K. & Suzuki, T.
  • Journal Title: Microscopy Research and Technique Volume: Vol.76,Issue4 Issue: 4 Pages: 342-349
  • DOI: 10.1002/jemt.22172
  • Peer Reviewed
• [Journal Article] Effects of plasma treatments on the controlled drug release from poly(ethylene-co-vinyl acetate) (2013)
  • Author(s): Hagiwara, K., Hasebe, T. & Hotta, A.
  • Journal Title: Surface and Coatings Technology Volume: Vol.216 Pages: 318-323
  • DOI: 10.1016/j.surfcoat.2012.11.064
  • Peer Reviewed
• [Journal Article] Controlled formation of wrinkled diamond-like carbon (DLC) film on grooved poly(dimethylsiloxane) substrate. (2012)
  • Author(s): Hasebe T*, Nagashima S*, Tsuya D, Horikoshi T, Ochiai M, Tanigawa S, Koide Y, Hotta A, Suzuki T. (* Hasebe T and Nagashima S equally contributed to this work.)
  • Journal Title: Diam Relat Mater Volume: 22 Pages: 48-51
  • Peer Reviewed
• [Journal Article] Human umbilical vein endothelial cell interaction with fluorine-incorporated amorphous carbon films (2012)
  • Author(s): Yoshimoto, Y., Hasebe, T., Nagashima, S., Kamijo A., Nakatani, T., Yamagami T., Kitamura, N. & Kitagawa T.
  • Journal Title: Japanese Journal of Applied Physics Volume: Vol.51 Issue: 9R Pages: 1-7
  • DOI: 10.1143/jjap.51.090129
  • Peer Reviewed
• [Journal Article] Tailoring surface topographies of polymers by using ion beam : Recent advances and the potential applications in biomedical and tissue engineering (2011)
  • Author(s): T.Hasebe, S.Nagashima, Y.Yoshimoto, A.Hotta, T.Suzuki
  • Journal Title: Nuclear Instruments and Methods in Physics Research Section B : Beam Interactions with Materials and Atoms Volume: (In press) Pages: 134-136
  • DOI: 10.1016/j.nimb.2011.08.066
  • Peer Reviewed
• [Presentation] 炭素系材料が開く新世代医療器具の開発 (2015)
  • Author(s): 長谷部光泉, 松本知博, 中村挙子, 大花 継頼 , 堀田篤, 鈴木哲也
  • Organizer: ナノカーボンバイオシンポジウム
  • Place of Presentation: 東京大学伊藤国際学術研究センター, 日本
  • Year and Date: 2015-02-20
• [Presentation] 医工産学連携による血管内治療デバイスの開発の取り組み-From bench to bedside- (2015)
  • Author(s): 長谷部光泉, 松本知博
  • Organizer: 第33回群馬IVR研究会
  • Place of Presentation: 群馬大学医学部臨床中講堂, 日本
  • Year and Date: 2015-02-06
• [Presentation] 炭素系材料が開く新世代医療器具の開発 (2014)
  • Author(s): 長谷部光泉, 松本知博, 堀田 篤, 鈴木哲也
  • Organizer: 第28回ダイヤモンドシンポジウム
  • Place of Presentation: 東京電機大学, 日本
  • Year and Date: 2014-11-19 – 2014-11-21
  • Invited
• [Presentation] 医工連携プロジェクトによる国内型血管内ステントの開発 (2014)
  • Author(s): 長谷部光泉, 松本知博, 堀田篤, 鈴木哲也
  • Organizer: 第17回日本栓子検出と治療学会
  • Place of Presentation: 福岡大学病院福大メディカルホール, 日本
  • Year and Date: 2014-10-04 – 2014-10-05
• [Presentation] Hydrogen-free amorphous carbon film: Antibacterial and antithrom bogenic biocompatible coating. International Conference on Diamond and Carbon Materials (Young Scholar Award受賞） (2014)
  • Author(s): Kanazawa H, Hasebe T, Kitagawa T, Matsumoto T, Suzuki T
  • Organizer: International Conference on Diamond and Carbon Materials 2014
  • Place of Presentation: Madrid, Spain
  • Year and Date: 2014-09-06 – 2014-09-12
• [Presentation] 冠動脈ステントへのDLC コーティングについて―優れた生体適合性コーティング― (2014)
  • Author(s): 長谷部光泉, 松本知博
  • Organizer: 第23回日本血管インターベンション治療学会学術集会
  • Place of Presentation: 名古屋国際会議場, 日本
  • Year and Date: 2014-07-24 – 2014-07-25
• [Presentation] 早期内皮化促進性および抗血栓性を有するbFGF溶出性冠動脈ステントシステムの開発（最優秀賞受賞） (2014)
  • Author(s): 尾藤健太, 長谷部光泉, 堀田篤
  • Organizer: 第37回日本バイオレオロジー学会年会
  • Place of Presentation: 大宮ソニックシティビル, 日本
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] Newly developed drug-eluting stent system: Non-thrombogenic 2-methacryloyloxyethyl phosphorylcholine polymer combined with micro-patterned diamond-like carbon film as a scaffold for complete endothelial cells proliferation. (2013)
  • Author(s): Kitagawa N, Hasebe T, Kitamura N, Nagashima S, Suzuki T
  • Organizer: International Conference on Diamond and Carbon Materials 2013
  • Place of Presentation: Riva Del Garda-Fiere congressi Spa, Riva Del Garda, Italy
• [Presentation] Newly developed drug-eluting stent (DES) system for cardiovascular diseases. (2013)
  • Author(s): Hasebe T, Kitagawa T, Nagashima S, Matsumoto T, Kitamura N, Hotta A, Suzuki T
  • Organizer: International Symposium on Atomic Level Characterizations for New Materials and Devices (ALC) 2013
  • Place of Presentation: Sheraton Kona, The Big Island, Hawaii, USA
  • Invited
• [Presentation] 医工連携プロジェクトによるIVRリサーチ：基礎研究から臨床研究へ (2013)
  • Author(s): 長谷部光泉
  • Organizer: 栃木県放射線医会
  • Place of Presentation: 那須赤十字病院，日本
  • Invited
• [Presentation] 細胞接着性制御可能なマイクロパターニングDLC－MPCポリマー複合材料：薬剤徐放性血管内留置デバイスへの応用可能性（最優秀賞受賞） (2013)
  • Author(s): 前川駿人，長谷部光泉，北川智也，鈴木哲也
  • Organizer: 第27回ダイヤモンドシンポジウム
  • Place of Presentation: 日本工業大学学友館，日本
• [Presentation] Controlling the drug release rate from electrospun phospholipid polymer nanofibers with micro-patterned diamond-like carbon (DLC) coating. (2013)
  • Author(s): Yoshida S, Hasebe T, Suzuki T, Hotta A.
  • Organizer: APS March Meeting 2013
  • Place of Presentation: Baltimore Convention Center, Baltimore, MD, USA
• [Presentation] Human umbilical vein endothelial cell interaction with phospholipid polymer nanofibers coated by micro-patterned diamond-like carbon (DLC). (2013)
  • Author(s): Yoshida S, Hasebe T, Suzuki T, Hotta A.
  • Organizer: APS March Meeting 2013.
  • Place of Presentation: Baltimore Convention Center, Baltimore, MD, USA
• [Presentation] 生体適合性に優れた国産DLCコーティングステントとその臨床成績 (2012)
  • Author(s): 長谷部光泉
  • Organizer: 第21回 日本心血管インターベンション治療学会・学術集会
  • Place of Presentation: 朱雀メッセ，新潟市，日本
  • Invited
• [Presentation] 医工連携プロジェクトによる次世代生体適合性血管内デバイスの開発 (2012)
  • Author(s): 長谷部光泉
  • Organizer: 第53回真空に関する連合講演会
  • Place of Presentation: 甲南大学ポートアイランドキャンパス，神戸市，日本
  • Invited
• [Presentation] Controlling the drug release rate from biocompatible polymers by changing plasma-treated area. (2012)
  • Author(s): Hagiwara K, Hasebe T, Asakawa R, Kamijo A, Suzuki T, Hotta A.
  • Organizer: 39th ICMCTF (International Conference on Metallurgical Coatings and Thin Films)
  • Place of Presentation: Town & Country Convention Center, San Diego, CA, USA
• [Presentation] Effects of argon plasma treatment on controlling the drug release rate from biocompatible polymers. (2012)
  • Author(s): Hagiwara K, Hasebe T, Suzuki T, Hotta A.
  • Organizer: 39th ICMCTF (International Conference on Metallurgical Coatings and Thin Films)
  • Place of Presentation: Town & Country Convention Center, San Diego, CA, USA
• [Presentation] Anti-bacterial property of Hydrogen-free amorphous carbon coatings on Ar plasma-pretreated polytetrafluoroethylene (PTFE) with a-C:H:F interlayer. (2012)
  • Author(s): Umemoto H, Hasebe T, Kasai R, Yoshimoto Y, Nagashima S, Hotta A, Suzuki T.
  • Organizer: 2012 MRS Fall Meeting & Exhibit
  • Place of Presentation: The Hynes Convention Center, Boston, MA, USA
• [Book] Diamond-Like Carbon Coated on Polymers for Biomedical Applications. Thin Films and Coatings in Biology, Biological medical physics, Biomedical Engineering Series. Nazarpour S (Ed.) (2013)
  • Author(s): Hotta A, Hasebe T (invited contrib)
  • Total Pages: 58
  • Publisher: Springer, USA