| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
A model biofilm could be formed inside polymer microcapsules fabricated by using a microfluidic device. Pseudomonas aeruginosa biofilm formation is regulated by bacterial cell-cell communication termed as quorum sensing (QS). By co-culturing quorum quenching bacteria with the QS bacteria inside polymer capsules, the QS could be effectively inhibited. The enzymatic hydrolysis of the QS signal can block the target gene expression that depends on the signal concentration. The hydrolase was overexpressed and purified from Escherichia coli, and then immobilized on the polymer matrix. It was demonstrated that the biofilm formation of P. aeruginosa was successfully inhibited only by coexisting immobilized hydrolase inside the capsule.
The model biofilm is applicable to the bioremediation. The effective method for biofilm inhibition was also developed by using model biofilm.
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