| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Based on the homology, a biosynthetic gene cluster for a bioactive compound was select from the genome of Lecanicillium sp. to be introduced into Aspergillus oryzae.
Two vectors harboring a terminal region of the gene cluster and a loxP site were inserted at the outside of the target gene cluster. The target gene cluster between two loxP sites was rescued in a circular fosmid by Cre recombinase from the genomic DNA. The fosmid was introduced into A. oryzae. The resultant transformant was cultivated, and the metabolites were analyzed, displaying novel compounds which did not exist in the metabolites of the host strain. As a result of analysis on the compounds, the compounds were proven to be derived from the introduced gene cluster.
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