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Fabrication and characterization of plasma-polymerized film/biomolecule interface and its application for biosensors

Research Project

Project/Area Number 24560965
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionShibaura Institute of Technology

Principal Investigator

MUGURUMA Hitoshi  芝浦工業大学, 工学部, 教授 (20309719)

Co-Investigator(Kenkyū-buntansha) KUDO Susumu  九州大学, 工学(系)研究科, 教授 (70306926)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsバイオセンサ / プラズマ重合 / カーボンナノチューブ / 細胞 / パターンニング / 酵素センサ / バイオチップ / ナノ材料 / グルコース酸化酵素 / グルコース脱水素酵素 / 酵素
Outline of Final Research Achievements

We focus on bionanointerface composed by plasma-polymerized organic thin film and protein molecule as a target of bionanointerface research. The purpose is the structural analysis of ten nanometer thick bionanointerface composed by plasma-polymerized film and biomolecule and the investigation of the relationship between its structure and material/device characteristics, then, orientate boosting the ability and expressing the new functionality. This bionanointerface is importantly positioned at biomaterials and biosensors supporting medicine. We have achieved the following two matters. (1) The highly sensitive biosensor with electronically-type sorted single-walled carbon nanotube, plasma-polymerized film, and biomolecule is presented. (2) Patterning of endothelial cells and hepatic stellate cells by two step plasma polymerization processes is presented.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (21 results)

All 2015 2014 2013 2012

All Journal Article (8 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (13 results)

  • [Journal Article] Adhesion and alignment of nonparenchymal cells onto a patterned surface with a two-step plasma polymerization process2015

    • Author(s)
      H. Muguruma, T. Hoshi, R. Fujita, T. Sumii, S. Kudo
    • Journal Title

      Plasma Processes and Polymers

      Volume: 未定

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Electronically type-sorted carbon nanotube-based electrochemical biosensors with glucose oxidase and dehydrogenase2015

    • Author(s)
      H. Muguruma, T. Hoshino, K. Nowaki
    • Journal Title

      ACS Applied Materials and Interfaces

      Volume: 7 Pages: 584-592

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Amperometric biosensor with composites of carbon nanotube, hexaamineruthenium(III)chloride, and plasma-polymerized film2013

    • Author(s)
      T. Hoshino, T. Inoue, H. Muguruma
    • Journal Title

      IEICE Transaction on Electronics

      Volume: E96-C Pages: 1536-1540

    • NAID

      130003385225

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] NADH Sensing Using Neutral Red Functionalized Carbon Nanotube/Plasma-polymerized Film Composite Electrode2012

    • Author(s)
      Tatsuya Hoshino, Hitoshi Muguruma
    • Journal Title

      IEICE Transaction on Electronics

      Volume: E95-C Pages: 1300-1303

    • NAID

      10031023457

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] ドライプロセスを基盤とするバイオナノ界面の作製評価およびバイオセンサへの応用2012

    • Author(s)
      六車仁志
    • Journal Title

      Chemical Sensors

      Volume: 28 Pages: 62-70

    • Related Report
      2012 Research-status Report
  • [Journal Article] 見る,聴く,触る-バイオセンサの動向2012

    • Author(s)
      六車仁志
    • Journal Title

      電気学会誌

      Volume: 132 Pages: 413-416

    • NAID

      10030777583

    • Related Report
      2012 Research-status Report
  • [Journal Article] Amperometric biosensor based on multilayer containing carbon nanotube, plasma-polymerized film, electron transfer mediator phenothiazine, and glucose dehydrogenase2012

    • Author(s)
      Tatsuya Hoshino, Shin-ichiro Sekiguchi, Hotoshi Muguruma
    • Journal Title

      Bioelectrochemistry

      Volume: 84 Pages: 1-5

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] NADH Sensing Using Neutral Red Functionalized Carbon Nanotube/Plasma-polymerized Film Composite Electrode2012

    • Author(s)
      Tatsuya Hoshino, Hitoshi Muguruma
    • Journal Title

      Electrochemistry

      Volume: 80 Pages: 85-87

    • NAID

      10031023457

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] カーボンナノチューブの電気物性が電気化学バイオセンサ特性に及ぼす影響2015

    • Author(s)
      野脇航平、星野達也、六車仁志
    • Organizer
      2015年電気化学会春季大会、第55回化学センサ研究会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2015-03-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 電気物性が分離されたカーボンナノチューブを用いる酵素センサの動作機構の解明2015

    • Author(s)
      野脇航平、星野達也、六車仁志
    • Organizer
      2015年春季第62回応用物理学関係連合講演会
    • Place of Presentation
      東海大学平塚校舎
    • Year and Date
      2015-03-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Patterning of Endothelial Cells and Hepatic Stellate Cells with Two Step Plasma-polymerized Processes2014

    • Author(s)
      H. Muguruma, T. Hoshino, R. Fujita, T. Sumii, S. Kudo
    • Organizer
      5th International Conference on Plasma Medicine (ICPM5)
    • Place of Presentation
      奈良公会堂
    • Year and Date
      2014-05-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Amperometric biosensor with electronic type-controlled single-walled carbon nanotube2014

    • Author(s)
      K. Nowaki, Y. Shimizu, T. Hoshino, H. Muguruma
    • Organizer
      8th International Symposium on Organic Molecular Electronics
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] mperometric biosensor based on multilayer containing glucose oxidase, carbon nanotube, plasma-polymerized film, and electron transfer mediator2014

    • Author(s)
      K. Nemoto, T. Naito, K. Nowaki, H. Muguruma
    • Organizer
      8th International Symposium on Organic Molecular Electronics
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Structure observation of enzyme embedded in plasma-polymerized film and its biosensor performance2014

    • Author(s)
      Y. Kase, H. Muguruma
    • Organizer
      ISPlasma2014 / IC-PLANTS2014
    • Place of Presentation
      名古屋
    • Related Report
      2013 Research-status Report
  • [Presentation] 二段階プラズマ重合法を用いる血管内被細胞および星細胞のパターニング2014

    • Author(s)
      六車仁志、星野達也、藤田陵介、隅井干城、工藤奨
    • Organizer
      2014年春季第61回応用物理学関係連合講演会
    • Place of Presentation
      神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] An electron transfer-mediated amperometric biosensor based on plasma-polymerized thin film2013

    • Author(s)
      Y. Kase, H. Muguruma
    • Organizer
      Bio4Apps 2013: International Conference on BioSensors, BioElectronics, BioMedical Devices, BioMEMS/NEMS and Applications 2013 & 5th Sensing Biology Symposium
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] カーボンナノチューブと電子伝達媒介物質を用いる酵素センサ動作の低電位化2013

    • Author(s)
      内藤晃誠、井上貴博、星野達也、六車仁志
    • Organizer
      2013年秋季第74回応用物理学会学術講演会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] 電気的性質の分離したカーボンナノチューブとグルコース酸化酵素を用いるアンペロメトリックセンサ2013

    • Author(s)
      野脇航平、星野達也、六車仁志
    • Organizer
      2013年秋季第74回応用物理学会学術講演会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] 電気物性が分離されたカーボンナノチューブとグルコース脱水素酵素を用いるバイオセンサ2013

    • Author(s)
      菅原篤、岩瀬祐介、星野達也、六車仁志
    • Organizer
      2013年秋季第74回応用物理学会学術講演会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] 大気圧プラズマ重合による有機薄膜の作製評価2013

    • Author(s)
      野村亮太、中村竜介、六車仁志
    • Organizer
      2013年秋季第74回応用物理学会学術講演会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] プラズマ重合膜と金属性カーボンナノチューブを用いるグルコースバイオセンサ2012

    • Author(s)
      星野達也、六車仁志
    • Organizer
      2012年度電子情報通信学会ソサイエティ大会
    • Place of Presentation
      富山大学
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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