| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Thermophilic enzymes are generally stable and potentially useful for industrial processes. However, a crucial drawback for their use is that they are often nearly inactive at moderate and low temperatures. I previously reported that a small change in side-chain volume of a non-polar residue that interacts with the adenine moiety of the coenzyme NAD enhances the catalytic efficiency of the thermostable 3-isopropylmalate dehydrogenase and lactate dehydrogenase from the extreme thermophile Thermus thermophiles. In this study, I produced mutants of the glucose-1-dehydrogenase from the hyperthermophile Sulfolobus tokodaii in which one of the coenzyme-binding, non-polar residues was replaced by another non-polar residue. Because the mutant showed the improved low-temperature activity, I conclude that a small volume change of a non-polar residue’s side chain that interacts with the adenine of NAD(P) in a dehydrogenase will provide a general method to improve its low temperature activity.
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