| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
It is important to keep pluripotency for stem cells (embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells and hematopietic stem cells) during their culture and to induce optimal lineages of tissue cells in regenerative medicine using stem cells. We prepared biomaterials having different elasticity/stiffness where cell adhesion molecules and oligopeptides (nanosegments) were grafted. We have identified the specific cell adhesion peptides and extracellular matrices (ECMs), which keep pluripotency of stem cells cultured on the surface grafted with the peptide of ECMs. We have also identified the optimal elasticity and cell adhesion peptides, which guide the differentiation of osteoblasts from stem cells where the stem cells were cultured on the biomaterials grafted with the cell adhesion peptides or ECMs and having the specific elasticity/stiffness.
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