Development of Fluorescent Probes for the Detection of Oxidative Stress Markers and Their Application to Medical Diagnostics
| Project/Area Number |
24560969
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
SUZUKI Yoshio 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (60321907)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | バイオセンサー / 酸化ストレスマーカー / センサー / 分子プローブ |
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| Outline of Final Research Achievements |
Design and synthesis of fluorescent probes for the detection of aldehyde-modified proteins were carried out based on the knowledge obtained by the development of fluorescent reagents for the detection of proteins. As a result, molecular probes for the detection of aldehyde-modified proteins constructed by binding site and fluorescent molecules were developed, and fluorescence enhancement was observed after reaction between fluorescent probes and aldehyde-modified proteins. Moreover, fluorescent gel images after electrophoresis enabled the monitoring of aldehyde-modified proteins.
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Report
(4 results)
Research Products
(14 results)
