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Thermal Stabilization Property of Proteins Adsorbed onto Biomass Charcoal Powder Prepared at Low Temperatures

Research Project

Project/Area Number 24561013
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Recycling engineering
Research InstitutionTokyo Metropolitan University

Principal Investigator

NORITOMI Hidetaka  首都大学東京, 都市環境科学研究科, 助教 (20237895)

Research Collaborator ISHIYAMA Reona  
IWAI Daiki  
ENDO Nobuyuki  
KAI Ryotaro  
KATO Satoru  
KURIHARA Shunichi  
SATO Kunimichi  
TAKEMOTO Tetsuya  
TANAKA Masahiko  
NISHIGAMI Junpei  
HISHINUMA Keito  
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords低温創製バイオマス炭 / タンパク質 / 熱安定化 / 吸着 / 固定化 / 残存活性 / リゾチーム / α-キモトリプシン / 熱安定性 / 植物バイオマス廃棄物
Outline of Final Research Achievements

The utilization of biomass for energies and functional materials is one of the most important challenges to establish a recycling society. We have found out that biomass charcoal powder (BCP), which is prepared from plant biomass waste by pyrolysis at low temperatures, is very useful as a carrier for the thermal stabilization of proteins. Proteins were effectively adsorbed onto BCP. The thermal stability of proteins adsorbed onto BCP was much greater than that of free proteins, and was strongly dependent upon the adsorption conditions such as solution pH, ionic strength, and temperature. Moreover, we have revealed the heat-resistant mechanism on the basis of the structure of proteins. On the other hand, the denaturation of proteins was efficiently inhibited in an organic solvent, which worked as a denaturant, by the adsorption of proteins onto BCP.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (26 results)

All 2015 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (22 results)

  • [Journal Article] Heat-resistant properties of α-chymotrypsin adsorbed onto biomass charcoal powder2014

    • Author(s)
      Hidetaka Noritomi, Shunichi Kurihara, Nobuyuki Endo and Satoru Kato
    • Journal Title

      J. Biomaterials Nanobiotechnol.

      Volume: 5 Issue: 03 Pages: 179-185

    • DOI

      10.4236/jbnb.2014.53021

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Adsorption of α-chymotrypsin on plant biomass charcoal2013

    • Author(s)
      Hidetaka Noritomi, Keito Hishinuma, Shyunichi Kurihara, Jumpei Nishigami, Tetsuya Takemoto, Nobuyuki Endo and Satoru Kato
    • Journal Title

      J.Surf. Eng. Mater. Adv. Technol.

      Volume: 3 Pages: 269-274

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Adsorption of Lysozyme on Biomass Charcoal Powder Prepared from Plant Biomass Wastes2013

    • Author(s)
      Hidetaka Noritomi, Daiki Iwai, Ryotaro Kai, Masahiko Tanaka and Satoru Kato
    • Journal Title

      JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

      Volume: 46 Issue: 3 Pages: 196-200

    • DOI

      10.1252/jcej.12we182

    • NAID

      10031159964

    • ISSN
      0021-9592, 1881-1299
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immobilization of lysozyme on biomass charcoal powder prepared from plant biomass wastes.2012

    • Author(s)
      Hidetaka Noritomi, Reona Ishiyama, Ryotaro Kai, Daiki Iwai, Masahiko Tanaka and Satoru Kato
    • Journal Title

      J. Biomaterials Nanobiotechnol.

      Volume: 3 Issue: 04 Pages: 446-451

    • DOI

      10.4236/jbnb.2012.34045

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Effect of adsorption condition on thermal stability of proteins adsorbed onto biomass charcoal powder2015

    • Author(s)
      Hidetaka Noritomi, Shunichi Kurihara, Nobuyuki Endo, Satoru Kato and Kazumi Uchiyama
    • Organizer
      SEE-Mie2015
    • Place of Presentation
      Mie
    • Year and Date
      2015-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低温創製竹炭吸着リゾチームの耐熱性に対する吸着条件の効果2015

    • Author(s)
      乗富秀富,栗原駿一,遠藤信行,加藤 覚, 内山一美
    • Organizer
      分離技術会年会2015
    • Place of Presentation
      川崎
    • Year and Date
      2015-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Catalytic activity of protease immobilized onto biomass charcoal prepared at low temperatures in nonaqueous solvents2014

    • Author(s)
      Junpei Nishigami, Hidetaka Noritomi, Nobuyuki Endo, and Satoru Kato
    • Organizer
      Active Enzyme Molecule 2014
    • Place of Presentation
      Toyama
    • Year and Date
      2014-12-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Thermal stability of enzymes adsorbed on plant biomass charcoal2014

    • Author(s)
      Shunichi Kurihara, Hidetaka Noritomi, Nobuyuki Endo, and Satoru Kato
    • Organizer
      Active Enzyme Molecule 2014
    • Place of Presentation
      Toyama
    • Year and Date
      2014-12-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Optimization of adsorption condition for thermal stabilization of protein adsorbed on plant biomass charcoal2014

    • Author(s)
      Shunichi Kurihara, Hidetaka Noritomi, Nobuyuki Endo, and Satoru Kato
    • Organizer
      The 10th International Conference on Separation Science and Technology
    • Place of Presentation
      Nara
    • Year and Date
      2014-11-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] Solvent effect on the activity of protease immobilized onto bamboo charcoal prepared at low temperatures2014

    • Author(s)
      Junpei Nishigami, Hidetaka Noritomi, Nobuyuki Endo, and Satoru Kato
    • Organizer
      The 10th International Conference on Separation Science and Technology
    • Place of Presentation
      Nara
    • Year and Date
      2014-11-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] バイオマス炭吸着タンパク質の熱安定性における吸着条件の最適化2014

    • Author(s)
      栗原駿一,乗富秀富,遠藤信行,加藤 覚
    • Organizer
      第4回CSJ化学フェスタ2014
    • Place of Presentation
      東京
    • Year and Date
      2014-10-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低温創製竹炭に固定化されたプロテアーゼの活性に対する溶媒効果2014

    • Author(s)
      西上純平,乗富秀富,遠藤信行,加藤 覚
    • Organizer
      第4回CSJ化学フェスタ2014
    • Place of Presentation
      東京
    • Year and Date
      2014-10-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] バイオマス炭吸着プロテアーゼの耐熱性2014

    • Author(s)
      乗富秀富,栗原駿一,遠藤信行,加藤 覚
    • Organizer
      第65回コロイドおよび界面化学討論会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] バイオマス炭吸着プロテアーゼの有機溶媒耐性2014

    • Author(s)
      乗富秀富,西上純平,遠藤信行,加藤 覚
    • Organizer
      第65回コロイドおよび界面化学討論会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Activity and stability of HEWL adsorbed onto plant biomass charcoal2013

    • Author(s)
      Hidetaka Noritomi, Reona Ishikawa, Daiki Iwai, Nobuyuki Endo, and Satoru Kato
    • Organizer
      Enzyme Engineering XXII
    • Place of Presentation
      Toyama
    • Related Report
      2013 Research-status Report
  • [Presentation] 低温創製バイオマス炭の構造と酵素タンパク質の吸着挙動2013

    • Author(s)
      乗富秀富,岩井大輝,菱沼慶人,遠藤信行,加藤 覚
    • Organizer
      分離技術会年会2013
    • Place of Presentation
      千葉
    • Related Report
      2013 Research-status Report
  • [Presentation] 水溶液中における低温創製バイオマス炭吸着α-キモトリプシンの触媒特性2013

    • Author(s)
      栗原駿一,乗富秀富,遠藤信行,加藤 覚
    • Organizer
      分離技術会年会2013
    • Place of Presentation
      千葉
    • Related Report
      2013 Research-status Report
  • [Presentation] 有機溶媒中における低温創製バイオマス炭吸着α-キモトリプシンの触媒活性2013

    • Author(s)
      西上純平,乗富秀富,遠藤信行,加藤 覚
    • Organizer
      分離技術会年会2013
    • Place of Presentation
      千葉
    • Related Report
      2013 Research-status Report
  • [Presentation] 卵白リゾチームの触媒特性に及ぼすバイオマス炭界面の影響2013

    • Author(s)
      乗富秀富,石山玲央奈,岩井大輝,遠藤信行,加藤 覚
    • Organizer
      64回コロイドおよび界面化学討論会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Research-status Report
  • [Presentation] 竹炭への固定化によるα-キモトリプシンの非水媒体中における触媒活性の向上2013

    • Author(s)
      西上純平,乗富秀富,遠藤信行,加藤 覚
    • Organizer
      第3回CSJ化学フェスタ
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 竹炭への固定化によるα-キモトリプシンの熱安定化2013

    • Author(s)
      栗原駿一,乗富秀富,遠藤信行,加藤 覚
    • Organizer
      第3回CSJ化学フェスタ
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] Thermostability of lysozyme immobilized on biomass charcoal powder.2012

    • Author(s)
      Hidetaka Noritomi, Ryotaro Kai, Masahiko Tanaka, and Satoru Kato
    • Organizer
      12th Japan-China-Korea Joint Symposium on Enzyme Engineering
    • Place of Presentation
      Kanazawa
    • Related Report
      2012 Research-status Report
  • [Presentation] 低温熱分解によるバイオマス炭の創製とタンパク質の吸着2012

    • Author(s)
      乗富秀富、岩井大輝、田中正彦、加藤 覚
    • Organizer
      化学工学会横浜大会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 植物バイオマス廃棄物を原料とした低温創製炭の構造とタンパク質の吸着特性2012

    • Author(s)
      乗富秀富、岩井大輝、田中正彦、加藤 覚
    • Organizer
      第26回日本吸着学会研究発表会
    • Place of Presentation
      つくば
    • Related Report
      2012 Research-status Report
  • [Presentation] 低温創製バイオマス炭吸着リゾチームの熱安定性

    • Author(s)
      乗富秀富、内田 雄、栗原駿一、西上純平、甲斐龍太郎、田中正彦、加藤 覚
    • Organizer
      酵素工学研究会第68回講演会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Thermostability of lysozyme immobilized on biomass charcoal powder.

    • Author(s)
      Hidetaka Noritomi, Ryotaro Kai, Masahiko Tanaka, and Satoru Kato
    • Organizer
      Proceedings of the International Symposium on Sustainable Urban Environment 2012
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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