Drosophila reproductive isolation caused by the interspecific incompatibility among nucleoporins

• Project/Area Number: 24570001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: University of Tsukuba
• Principal Investigator: SAWAMURA Kyoichi 筑波大学, 生命環境系, 准教授 (90247205)
• Co-Investigator(Kenkyū-buntansha): MATSUNO Kenji 大阪大学, 大学院理学研究科, 教授 (60318227) ; ISHIKAWA Hiroyuki 千葉大学, 大学院理学研究科, 准教授 (00398819)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 生殖的隔離 / ショウジョウバエ / 核膜孔 / ヌクレオポリン / 雑種致死 / 雑種不妊 / 遺伝的不和合 / 種分化 / 遺伝学 / 進化

Outline of Final Research Achievements

Nuclear pore proteins from Drosophila simulans cause reproductive isolation in D. melanogaster. The present study clarified (1) two scafold nucleoporins (Nup160 and Nup96) interact each other, (2) these two nucleoporins are necessary but not enough to cause hybrid inviability, and (3) Nup160 alone causes hybrid female sterility but Nup96 does not. Thus, genetic system of hybrid incompatibility is not simple.

Research Products

• [Journal Article] Allelic assymmetry of the Lethal hybrid rescue (Lhr) gene expression in the htbrid between Drosophila melanogaster and D. simulans: confirmation by using genetic variation of D. melanogaster (2014)
  • Author(s): M.Shirata, Q.Araye, K.Maehara, S.Enya, T.Takano-Shimizu, K.Sawamura
  • Journal Title: Genetica Volume: 142 Pages: 43-48
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A test of double interspecific introgression of nucleoporin genes in Drosophila (2014)
  • Author(s): K.Sawamura, K.Maehara, Y.Keira, H.O.Ishikawa, T.Sasamura. T.Yamakawa, K.Matsuno
  • Journal Title: G3 Genes, Genomes, Genetics Volume: 4 Issue: 11 Pages: 2101-2106
  • DOI: 10.1534/g3.114.014027
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Allelic asymmetry of the Lethal hybrid rescue (Lhr) gene expression in the hybrid between Drosophila melanogaster and D. simulans : confirmation by using genetic variations of D. melanogaster (2014)
  • Author(s): Shirata, M., Araye, Q., Maehara, K., Enya, S., Takano-Shimizu, T., and Sawamura, K.
  • Journal Title: Genetica Volume: 142 Issue: 1 Pages: 43-48
  • DOI: 10.1007/s10709-013-9752-3
  • Peer Reviewed
• [Journal Article] アナナスショウジョウバエ類における種間遺伝子浸透の分子生物学的証拠：ペナン島（マレーシア）の事例 (2014)
  • Author(s): 澤村京一、佐藤玄、李昭揚、上村佳孝、松田宗男
  • Journal Title: 昆虫DNA研究会ニュースレター Volume: 20 Pages: 41-44
• [Journal Article] Genetic decay of balancer chromosomes in Drosophila melanogaster (2013)
  • Author(s): Q.Araye, K.Sawamura
  • Journal Title: Fly Volume: 7 Issue: 3 Pages: 184-186
  • DOI: 10.4161/fly.24466
  • Peer Reviewed
• [Journal Article] Mechanisms of Speciation (2012)
  • Author(s): K.Sawamura, C.T.Ting, A.Kopp, L.C.Moyle
  • Journal Title: International Journal of Evolutionary Biology Volume: 2012 Pages: 1-2
  • DOI: 10.1155/2012/820358
• [Journal Article] Chromatin evolution and molecular drive in speciation (2012)
  • Author(s): K.Sawamura
  • Journal Title: International Journal of Evolutionary Biology Volume: 2012 Pages: 1-9
  • DOI: 10.1155/2012/301894
  • Peer Reviewed
• [Journal Article] Genetic dissection of <i>Nucleoporin 160</i> (<i>Nup160</i>), a gene involved in multiple phenotypes of reproductive isolation in <i>Drosophila</i> (2012)
  • Author(s): K.Maehara, T.Murata, N.Aoyama, K.Matsuno, K.Sawamura
  • Journal Title: Genes & Genetic Systems Volume: 87 Issue: 2 Pages: 99-106
  • DOI: 10.1266/ggs.87.99
  • NAID: 10030616563
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed
• [Presentation] ショウジョウバエにおけるヌクレオポリン遺伝子の種間イントログレッション (2014)
  • Author(s): 澤村京一、前原一慶、計良陽子、石川裕之、笹村剛司、山川智子、松野建治
  • Organizer: 日本遺伝学会
  • Place of Presentation: 長浜バイオ大学、長浜
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] キイロショウジョウバエの実験室系統における集団サイズと近交弱勢 (2014)
  • Author(s): 新井健太、澤村京一
  • Organizer: 日本遺伝学会
  • Place of Presentation: 長浜バイオ大学、長浜
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] Population size and inbreeding depression in Drosophila melanogaster lab strains (2014)
  • Author(s): Q.Araye, K.Sawamura
  • Organizer: Japanese Drosophila research Conference
  • Place of Presentation: Kanazawa Theatre, Kanazawa
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] Analysis of genes suppressing the Lethal hybrid rescue between Drosophila melanogaster and D. simulans (2013)
  • Author(s): M.Shirata, Q.Araye, K.Maehara, K.Sawamura
  • Organizer: International Congress of Genetics
  • Place of Presentation: The Sands Expo and Convention Center, Singapore
• [Presentation] アナナスショウジョウバエ類における種間遺伝子浸透の分子生物学的証拠：ペナン島（マレーシア）の事例 (2013)
  • Author(s): 澤村京一、佐藤玄、李昭揚、上村佳孝、松田宗男
  • Organizer: 昆虫DNA研究会
  • Place of Presentation: 東京大学、東京
• [Presentation] 遺伝的組換えを失った染色体の運命 (2013)
  • Author(s): 新井健太、澤村京一
  • Organizer: 日本進化学会
  • Place of Presentation: 筑波大学、つくば
• [Presentation] アナナスショウジョウバエ類の野外集団における一方向的な種間浸透：ペナン島（マレーシア）の事例 (2013)
  • Author(s): 澤村京一、佐藤玄、李昭揚、上村佳孝、松田宗男
  • Organizer: 日本進化学会
  • Place of Presentation: 筑波大学、つくば
• [Presentation] ショウジョウバエ雑種致死救済効果を抑制する遺伝子の解析 (2013)
  • Author(s): 白田美香、新井健太、前原一慶、塩谷天、高野敏行、澤村京一
  • Organizer: 日本遺伝学会
  • Place of Presentation: 慶應大学、日吉
• [Presentation] Genetic decay of balancer chromosomes in Drosophila melanogaster (2012)
  • Author(s): Q.Araye, K.Sawamura
  • Organizer: Japanese Drosophila Research Conference
  • Place of Presentation: 慈恵医大（東京）
• [Presentation] A gene causing multiple phenotypes of hybrid incompatibility in Drosophila: Nucleoporin 160 (Nup160) (2012)
  • Author(s): K.Maehara, K.Sawamura
  • Organizer: Japanese Drosophila Research Conference
  • Place of Presentation: 慈恵医大（東京）
• [Presentation] Suppressor hunting for lethal hybrid rescue between Drosophila melanogaster and D. simulans (2012)
  • Author(s): M.Shirata, K.Sawamura
  • Organizer: Japanese Drosophila Research Conference
  • Place of Presentation: 慈恵医大（東京）
• [Presentation] アナナスショウジョウバエ類野外集団における種間浸透：ペナン島（マレーシア）の事例
  • Author(s): 澤村京一、佐藤玄、李昭揚、上村佳孝、松田宗男
  • Organizer: ショウジョウバエ多様性研究会シンポジウム
  • Place of Presentation: 国立遺伝学研究所、三島
  • Invited
• [Presentation] アナナスショウジョウバエ類を用いた種分化の研究
  • Author(s): 澤村京一・松田宗男
  • Organizer: 日本遺伝学会第84回大会 ワークショップ「ショウジョウバエの進化・集団遺伝学」
  • Place of Presentation: 九州大学（福岡）
  • Invited
• [Remarks] 筑波大学進化学教室
  • URL: http://www.biol.tsukuba.ac.jp/~sawamura/
• [Remarks] 筑波大学進化遺伝学教室
  • URL: http://www.biol.tsukuba.ac.jp/~sawamura/
• [Remarks] 筑波大学進化遺伝学教室
  • URL: http://www.biol.tsukuba.ac.jp/~sawamura/