| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Two strains of Kanzawa spider mite (KSM) - the white scar strain (W-strain) and the red scar strain (R-strain) - induce distinct defense response in the same Phaseolus leaves. In this study, we carried out RNAseq-based transcriptome analysis for mRNA of R-strain and W-strain on kidney bean plants (P. vulgaris) to identify KSM genes expressed specifically in either R-strain or W-strain. Of particular interest is the fact that some genes involved in the detoxification system in KSMs were expressed higher in R-strains than in W-strains. Some of detoxification enzyme activities were higher in R-strain than in W-strains as well. Further we investigated whether the differences in detoxification affect the tolerance of acaricides. R-strain showed less mortality than W-strain when they were treated with 3 out of 5 acaricides, suggesting that R-strain latently possesses higher detoxification ability than W-strain.
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