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Molecular interactions at plant-insect interface

Research Project

Project/Area Number 24570026
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ecology/Environment
Research InstitutionOkayama University

Principal Investigator

GALIS Ivan  岡山大学, 資源植物科学研究所, 教授 (90360502)

Co-Investigator(Kenkyū-buntansha) SHINYA Tomonori  岡山大学, 資源植物科学研究所, 助教 (80514207)
TANI Akio  岡山大学, 資源植物科学研究所, 准教授 (00335621)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords防御応答 / 昆虫エリシター / 植食生昆虫 / ジャスモン酸 / Oryza sativa / ファイトアレキシン / イネ / 傷害 / rice / phenolamide / phytoalexin / jasmonic acid / defense / elicitor / ホルモン / 昆虫 / 代謝物 / エリシター / 植食性昆虫
Outline of Final Research Achievements

We established a novel system to study rice-insect interactions. We showed that the accumulation of active defense hormone jasmonoyl-L-isoleucine (JA-Ile) in rice depends on the function of OsJAR1 enzyme. JA and JA-Ile accumulations in rice were controlled by the availability of the main pathway substrate, α-linolenic acid. In comparison to wounding, rice plants specifically responded to elicitors present in chewing herbivore oral secretions. We identified two novel herbivory-related phenolamide phytoalexins. Using ROS and rice phenolamides as molecular markers, we identified and partially purified a new putative insect elicitor from Mythimna loreyi oral secretions.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (16 results)

All 2015 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Invited: 3 results) Remarks (1 results)

  • [Journal Article] α-Linolenic acid concentration and not wounding per se is the key regulator of octadecanoid (oxylipin) pathway activity in rice (Oryza sativa L.)leaves2014

    • Author(s)
      Christeller, J.T., Galis, I.
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 83 Pages: 117-125

    • DOI

      10.1016/j.plaphy.2014.07.013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Response of rice to insect elicitors and the role of OsJAR1 in wound and herbivory-induced JA-Ile accumulation.2013

    • Author(s)
      Fukumoto K, Alamgir K Md, Yamashita Y, Mori IC, Matsuura H and Galis I
    • Journal Title

      Journal of Integrative Plant Biology

      Volume: 55 Issue: 8 Pages: 775-784

    • DOI

      10.1111/jipb.12057

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Characterization of herbivore-induced rice volatiles under real and mimic herbivory2015

    • Author(s)
      Sobhy I.S., Miyake A., Galis I.
    • Organizer
      Annual Meeting of JSPP 2015
    • Place of Presentation
      Tokyo
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of rice protection strategies based on the natural defense mechanisms against herbivores2015

    • Author(s)
      Tanabe T., Hojo Y., Shinya T., Alamgir Md. K., Galis I.
    • Organizer
      Annual Meeting of JSPP 2015
    • Place of Presentation
      Tokyo
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Isolation of novel insect elicitors from rice herbivores2015

    • Author(s)
      Shinya T., Hojo Y., Alamgir Md. K., Desaki Y., Shibuya N., Galis I.
    • Organizer
      Annual Meeting of JSPP 2015
    • Place of Presentation
      Tokyo
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of novel plant protection strategies based on the natural defense mechanisms against herbivores in rice2014

    • Author(s)
      Galis, I.
    • Organizer
      The 1st Okayama University and King Faisal University’s Workshop on Environmental and Life Science
    • Place of Presentation
      Okayama
    • Year and Date
      2014-11-13 – 2014-11-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 植食性昆虫に対するイネの防御応答および認識機構の解析2014

    • Author(s)
      新屋友規
    • Organizer
      岡山大学資源植物科学研究所創立100周年記念シンポジウム
    • Place of Presentation
      倉敷
    • Year and Date
      2014-10-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] First steps towards understanding of rice defense mechanisms against herbivores2014

    • Author(s)
      Galis I, Alamgir K Md, Miyake A, Fukumoto K, Hojo Y
    • Organizer
      Annual Meeting of JSPP 2014
    • Place of Presentation
      Toyama
    • Related Report
      2013 Research-status Report
  • [Presentation] Rice defense against herbivores: what are the metabolites?2013

    • Author(s)
      Galis I, Alamgir K Md
    • Organizer
      International Chemical Ecology Conference
    • Place of Presentation
      Melbourne, AUSTRALIA
    • Related Report
      2013 Research-status Report
  • [Presentation] The role of jasmonoyl-L-isoleucine synthase OsJAR1 in rice defense against herbivores2013

    • Author(s)
      Fukumoto K, Alamgir K Md, Yamashita Y, Galis I
    • Organizer
      International Chemical Ecology Conference
    • Place of Presentation
      Melbourne, AUSTRALIA
    • Related Report
      2013 Research-status Report
  • [Presentation] Rice defense against herbivores2013

    • Author(s)
      Galis I, Alamgir K Md
    • Organizer
      54th Annual Meeting of the Japanese Society of Plant Physiologists
    • Place of Presentation
      Okayama, JAPAN
    • Related Report
      2012 Research-status Report
  • [Presentation] Functional characterization of jasmonoyl-L-isoleucine synthase (OsJAR1) mutant rice plants2013

    • Author(s)
      Fukumoto K, Yamashita Y, Alamgir K Md, Galis I
    • Organizer
      54th Annual Meeting of the Japanese Society of Plant Physiologists
    • Place of Presentation
      Okayama, JAPAN
    • Related Report
      2012 Research-status Report
  • [Presentation] Constitutive defense in finger millet against whitebacked planthopper and inducible defenses in rice plants against brown planthopper2013

    • Author(s)
      Alamgir K Md, Galis I, Kim C-S
    • Organizer
      54th Annual Meeting of the Japanese Society of Plant Physiologists
    • Place of Presentation
      Okayama, JAPAN
    • Related Report
      2012 Research-status Report
  • [Presentation] Contribution of metabolomics and transcriptomics to understanding of plant defense against herbivores2012

    • Author(s)
      Galis I, Baldwin IT
    • Organizer
      XXIV International Congress of Entomology
    • Place of Presentation
      Daegu, KOREA
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Metabolic and structural changes in plants exposed to biotic stress conditions

    • Author(s)
      Galis I
    • Organizer
      The 6th Bio-energy & Biotechnology Symposium
    • Place of Presentation
      Gwangju, KOREA
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks] 岡山大学 資源植物科学研究所 植物昆虫間相互作用グループ

    • URL

      http://www.rib.okayama-u.ac.jp/PIN/index.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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