| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We have previously reported that reactive oxygen species (ROS) act primarily by inhibiting the repair of photosystem II (PSII) during photoinhibition; that ROS suppress the synthesis de novo of proteins that are necessary for the repair of PSII; and that translation factor EF-G is a target of ROS. In the present study, we found that another translation factor EF-Tu is also a target of ROS, being inactivated via formation of an intermolecular disulfide bond and sulfenic acid at Cys82 in the cyanobacterium Synechocystis sp PCC 6803; that expression of EF-Tu in which Cys82 had been replaced by serine rendered Synechocystis cells more resistant against photoinhibition of PSII; and that chloroplast-localized EF-G and EF-Tu from Arabidopsis thaliana are also sensitive to oxidation by ROS.
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