Effects of phospholipid biosynthesis mutation on the mitochondrial respiratory activity at ambient and low temperatures

• Project/Area Number: 24570040
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: NISHIDA Ikuo 埼玉大学, 理工学研究科, 教授 (40189288)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ホスファチジルエタノールアミン / ミトコンドリア内膜 / 呼吸活性 / シトクロムオキシダーゼ / 葉の発達 / 葉の呼吸活性 / リン脂質組成 / pect1-4 / AOX呼吸活性 / 早期花成 / cct1-2 / ap3様ホメオティック変異 / 低温感受性 / 形成葉分化促進 / 植物 / リン脂質 / 呼吸 / シトクロムｃオキシダーゼ / 小胞体油脂生合成 / 種子貯蔵タンパク質

Outline of Final Research Achievements

Mitochondria are important organelles for respiratory activity in plants. In this study, the role of phosphatidylethanolamine, which is the major non-bilayer phospholipid of mitochondrial inner membrane, is investigated using Arabidopsis pect1-4 mutants, which exhibit one fourth of the CTP:phosphorylethanolamine cytidylyltransferase activity. We found that the leaf respiratory activity increases with rosette leaf development in the wild type, but it did not increase in pect1-4 mutants under prolonged short-day growth conditions. Cytochrome oxidase activity was selectively inhibited in pect1-4 mutants, with no change in COXII protein levels. In pect1-4 mutants, PE levels decreased in isolated mitochondria compared with that in wild-type mitochondrial. These results suggested that a shortage of phosphatidylethanolamine in the mitochondrial inner membrane causes a partial inhibition of COX activity.

Research Products

• [Journal Article] Epigenetic floral homeotic mutation in pD991-AP3-derived T-DNA-tagged lines for CTP:phosphorylcholine Cytidylyltransferase (CCT) genes: the homeotic mutation of the cct1-1 allele is enhanced by the cct2 allele and alleviated by CCT1 overexpression. (2015)
  • Author(s): Hayakawa, Y., Duan, Z., Yadake, M., Tsukano, J., Yamaoka, Y., Inatsugi, R., Fujiki, Y. Oikawa, A., Saito, K. and Nishida, I.
  • Journal Title: J. Plant Biol. Volume: 58 Issue: 3 Pages: 183-192
  • DOI: 10.1007/s12374-014-0587-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mitochondrial phosphatidylethanolamine level modulates cytochrome c oxidase activity to maintain respiration capacity in Arabidopsis thaliana rosette leaves. (2013)
  • Author(s): Otsuru, M., Yanbo, Y., Mizoi, J., Kawamoto–Fujioka, M., Wang, J., Fujiki, Y. and Nishida, I.
  • Journal Title: Plant Cell Physiology Volume: 54 Issue: 10 Pages: 1612-1619
  • DOI: 10.1093/pcp/pct104
• [Journal Article] Rapid induction of lipid droplets in Chlamydomonas reinhardtii and Chlorella vulgaris by brefeldin A. (2013)
  • Author(s): Kim, S., Kim, H., Ko, D., Yamaoka, Y., Otsuru, M., Kawai-Yamada, M., Ishikawa, T., Oh, H.-M., Nishida, I., Li-Beisson, Y., and Lee, Y.
  • Journal Title: PLOS one Volume: 8 Issue: 12 Pages: 295-304
  • DOI: 10.1371/journal.pone.0081978
  • Peer Reviewed
• [Journal Article] The ABCA9 transporter facilitates seeds storage lipid synthesis at the endoplasmic reticulum. (2013)
  • Author(s): Kim, S., Yamaoka, Y., Ono, H., Kim, H., Shim, D., Maeshima, M., Martinoia, E., Cahoon, E.B., Nishida, I., Lee, Y.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 110 Issue: 2 Pages: 773-778
  • DOI: 10.1073/pnas.1214159110
  • Peer Reviewed
• [Journal Article] Genetic disruption of CRC 12S globulin increases seed oil content and seed yield in Arabidopsis thaliana. (2013)
  • Author(s): Fujiki, Y., Kudo, K., Ono, H., Otsuru, M., Yasuyo Yamaoka, Y., Akita, M. and Nishida, I.
  • Journal Title: Plant Biotech. Volume: 000
  • NAID: 10031202052
  • Peer Reviewed
• [Presentation] シロイヌナズナのpect1-4変異はシアン耐性呼吸変異aox1a-1と合成致死性を示す (2015)
  • Author(s): 矢竹美樹、ユヤンボ、藤木友紀、野口航、西田生郎
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都・世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] A mechanism of early flowering in pect1-4 mutants of Arabidopsis thaliana (2015)
  • Author(s): Natsumi Hoshino,Saki Ikegai,Miki Yadake,Mayu Nakagawa,Yuki Fujiki,Ikuo Nishida
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都・世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Ikuo Nishida, Saki Ikegai, Miki Yadake, Natsumi Hoshino, Mayu Nakagawa, Yuki Fujiki (2015)
  • Author(s): The mechanism of early flowering in pect1-4 mutants-A new hypothesis
  • Organizer: Gordon Research Conference
  • Place of Presentation: Galvestone, USA
  • Year and Date: 2015-02-01 – 2015-02-06
• [Presentation] Combination of the genetic disruption of 12S globulins and the overexpression of BnDGAT1 improves seed oil productivity in Arabidopsis thaliana (2014)
  • Author(s): Yuki Fujiki,Ai Inanobe,Kazumasa Kudo,Asuka Kato,Ikuo Nishida
  • Organizer: ISPL 21th International Symposium on Plant Lipids
  • Place of Presentation: University of Guelph (Canada)
  • Year and Date: 2014-07-10 – 2014-07-14
• [Presentation] Characterization of axillary bud formation in Arabidopsis pect1-4 mutants (2014)
  • Author(s): Saki Ikegai,Miki Yadake,Masumi Otsuru,Mayu Nakagawa,Yuki Fujiki,Ikuo Nishida
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] シロイヌナズナpect1-4変異体が示す早期花成の誘導メカニズム (2014)
  • Author(s): 矢竹美樹、生貝咲貴、大鶴真寿美、中川繭、藤木友紀、西田生郎
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] シロイヌナズナpect1-4変異は自律的経路を介して早期花成を誘導する (2013)
  • Author(s): 生貝咲貴、矢竹美樹、大鶴真寿美、中川繭、藤木友紀、西田生郎
  • Organizer: 第２６回植物脂質シンポジウム
  • Place of Presentation: 北海道大学
• [Presentation] シロイヌナズナにおけるpect1-4変異がもたらすミトコンドリアの脂質組成と呼吸活性への影響 (2013)
  • Author(s): 大鶴真寿美、Yu Yanbo, 溝井順哉、藤木友紀、西田生郎
  • Organizer: 第26回植物脂質シンポジウム
  • Place of Presentation: 北海道大学
• [Presentation] シロイヌナズナにおけるcct変異はスフィンゴ脂質代謝を活性化し、稔性に影響を及ぼす (2013)
  • Author(s): 矢竹美樹、相澤貴志、石川寿樹、藤木友紀、川合真紀、長谷川登志夫、西田生郎
  • Organizer: 第26回植物脂質シンポジウム
  • Place of Presentation: 北海道大学
• [Presentation] Phosphatidylserine is involved in endocytic vesicles and important for phragmoplast formation in Arabidopsis thaliana (2013)
  • Author(s): Y Yamaoka,Y Lee,I Nishida
  • Organizer: 2013 Annual Meeting of the Korean Society of Plant Biologists & the 5th Asian Symposium on Plant Lipids
  • Place of Presentation: Gwangju
• [Presentation] Genetic disruption of 12S globulin genes increases seed oil content and seed yield in Arabidopsis thaliana (2013)
  • Author(s): Y Fujiki,K Kudo,A Kato,I Nishida
  • Organizer: 2013 Annual Meeting of the Korean Society of Plant Biologists & the 5th Asian Symposium on Plant Lipids
  • Place of Presentation: Gwangju
• [Presentation] Physiological significance of the phosphatidylethanolamine biosynthesis in Arabidopsis thaliana (2013)
  • Author(s): Nishida, I.
  • Organizer: 16th International Workshop on Plant Membrane Biology (IWPMB2013)
  • Place of Presentation: 倉敷芸文館
  • Invited
• [Presentation] pect1-4 mutation affects the cytochorome oxidase pathway capacity of mitochondrial respiration in Arabidopsis thaliana (2012)
  • Author(s): Otsuru, M., Yu, Y., Fujiki, Y. and Nishida, I.
  • Organizer: 20th International Symposium on Plant Lipids (ISPL2012)
  • Place of Presentation: Seville, Spain
• [Remarks] Nishida Lab Publication
  • URL: http://www.molbiol.saitama-u.ac.jp/~nishida/Nishida_Lab/Publication.html
• [Remarks] 国際共同研究が拓く油脂増産技術 －植物脂肪酸を油脂合成の場に運ぶしくみを解明－
  • URL: http://www.molbiol.saitama-u.ac.jp/~nishida/Nishida_Lab/Pub56.html