| Project/Area Number |
24570040
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Saitama University |
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Principal Investigator |
NISHIDA Ikuo 埼玉大学, 理工学研究科, 教授 (40189288)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ホスファチジルエタノールアミン / ミトコンドリア内膜 / 呼吸活性 / シトクロムオキシダーゼ / 葉の発達 / 葉の呼吸活性 / リン脂質組成 / pect1-4 / AOX呼吸活性 / 早期花成 / cct1-2 / ap3様ホメオティック変異 / 低温感受性 / 形成葉分化促進 / 植物 / リン脂質 / 呼吸 / シトクロムｃオキシダーゼ / 小胞体油脂生合成 / 種子貯蔵タンパク質 |
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| Outline of Final Research Achievements |
Mitochondria are important organelles for respiratory activity in plants. In this study, the role of phosphatidylethanolamine, which is the major non-bilayer phospholipid of mitochondrial inner membrane, is investigated using Arabidopsis pect1-4 mutants, which exhibit one fourth of the CTP:phosphorylethanolamine cytidylyltransferase activity. We found that the leaf respiratory activity increases with rosette leaf development in the wild type, but it did not increase in pect1-4 mutants under prolonged short-day growth conditions. Cytochrome oxidase activity was selectively inhibited in pect1-4 mutants, with no change in COXII protein levels. In pect1-4 mutants, PE levels decreased in isolated mitochondria compared with that in wild-type mitochondrial. These results suggested that a shortage of phosphatidylethanolamine in the mitochondrial inner membrane causes a partial inhibition of COX activity.
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