Regulatory mechanism in metabolic allocation of nitrogen-containing compounds from central metabolisms to specialized metabolisms in plants

• Project/Area Number: 24570041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Chiba University
• Principal Investigator: YAMAZAKI Mami 千葉大学, 薬学研究科(研究院), 准教授 (70222370)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: アルカロイド / アミノ酸 / リジン / オルニチン / トリプトファン / 特異代謝 / 二次代謝 / ポリアミン / リジン脱炭酸酵素 / 代謝アロケーション / アミン酸化酵素 / 代謝 / 脱炭酸酵素

Outline of Final Research Achievements

Most of plant alkaloids are produced from amino acids. In this study, the molecular basis of metabolite allocation from amino acid metabolism (central metabolism) to alkaloid biosynthesis (specialized metabolism) was investigated. Quinolizidine alkaloids (QA) and lycopodium alkaloids (LA) are derived from lysine. is lysine decarboxylation. The cDNAs encoding lysine/ornithine decarboxylase (L/ODC) catalyzing the first step QA and LA biosynthesis were isolated from several species and characterized using recombinant protein expressed in E. coli. The enzymes from QA/LA-producing plants exhibited relatively higher lysine-decarboxylase activities comparing to those of other species. It is suggested that this difference leads the change in metabolic allocation from lysine metabolism to alkaloid biosynthesis.

Research Products

• [Journal Article] Revisiting anabasine biosynthesis in tobacco hairy roots expressing plant lysine decarboxylase gene by using ¹⁵N-labeled lysine (2014)
  • Author(s): Somnuk Bunsupa, Kana Komastsu, Ryo Nakabayashi, Kazuki Saito, and Mami Yamazaki
  • Journal Title: Plant Biotechnology Volume: 31 Issue: 5 Pages: 511-518
  • DOI: 10.5511/plantbiotechnology.14.1008a
  • NAID: 130004721174
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Coupling deep transcriptome analysis with untargeted metabolic profiling in Ophiorrhiza pumila to further the understanding of the biosynthesis of the anti-cancer alkaloid camptothecin and anthraquinones (2013)
  • Author(s): Mami Yamazaki, Keiichi Mochida, Takashi Asano, Ryo Nakabayashi, Motoaki Chiba, Udomson Nirin, Yasuyo Yamazaki, Dayan B. Goodenowe, Ushio Sankawa, Takuhiro Yoshida, Atsushi Toyoda, Yasushi Totoki, Yoshiyuki Sakaki, Elsa Góngora-Castill, C. Robin Buell, Tetsuya Sakurai and Kazuki Saito
  • Journal Title: Plant Cell Physiol. Volume: 54 Issue: 5 Pages: 686-696
  • DOI: 10.1093/pcp/pct040
  • NAID: 10031177048
  • Peer Reviewed
• [Journal Article] Suppression of camptothecin biosynthetic genes results in metabolic modification of secondary products in hairy roots of Ophiorrhiza pumila (2013)
  • Author(s): Takashi Asano, Kanae Kobayashi, Emi Kashihara, Hiroshi Sudo, Ryosuke Sasaki, Yoko Iijima, Koh Aoki, Daisuke Shibata, Kazuki Saito, Mami Yamazaki
  • Journal Title: Phytochemistry Volume: 91 Pages: 128-139
  • DOI: 10.1016/j.phytochem.2012.04.019
  • Peer Reviewed
• [Journal Article] Lysine decarboxylase catalyzes the first step of quinolizidine alkaloid biosynthesis and coevolved with alkaloid production in Leguminosae (2012)
  • Author(s): Somnuk Bunsupa, Kae Katayama, Emi Ikeura, Akira Oikawa, Kiminori Toyooka, Kazuki Saito, Mami Yamazaki.
  • Journal Title: Plant Cell Volume: 24 Issue: 3 Pages: 1202-1216
  • DOI: 10.1105/tpc.112.095885
  • Peer Reviewed
• [Journal Article] Quinolizidine alkaloid biosynthesis: recent advances and future prospects (2012)
  • Author(s): Somnuk Bunsupa, Mami Yamazaki and Kazuki Saito
  • Journal Title: Frontiers in Pant Science Volume: 3 Pages: 239-239
  • DOI: 10.3389/fpls.2012.00239
  • Peer Reviewed
• [Presentation] アミノ酸からアルカロイド生合成への代謝アロケーションの遺伝子エンジニアリング (2015)
  • Author(s): 山崎真巳、小松可奈、Somnuk Bunsupa, 斉藤和季
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] ヒカゲノカズラのリジン／オルニチン脱炭素酵素の局在解析．，21A-01．（平成26年8月21-22日．盛岡） (2014)
  • Author(s): 小松可奈，Somnuk Bunsupa，斉藤和季，山崎真巳
  • Organizer: 第32回日本植物細胞分子生物学会（盛岡）大会・シンポジウム
  • Place of Presentation: 盛岡
  • Year and Date: 2014-08-21 – 2014-08-22
• [Presentation] Cloning and Characterization of copper amine oxidase from quinolizidine-alkaloid producing Lupinus angustifolius. 21B-15． (2014)
  • Author(s): Bunsupa Somnuk, Aoyagi Kaori, Saito Kazuki, Yamazaki Mami
  • Organizer: 第32回日本植物細胞分子生物学会（盛岡）大会・シンポジウム
  • Place of Presentation: 盛岡
  • Year and Date: 2014-08-21 – 2014-08-22
• [Presentation] Cloning and characterization of copper amine oxidase from quinolizidine-alkaloid accumulating Lupinus angusutifolius (2014)
  • Author(s): Somnuk Bunsupa, Kaori Aoyagi, Kazuki Saito, Mami Yamazaki
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山
• [Presentation] Gene Mining for Camptothecin Production in Ophiorrhiza pumila (2013)
  • Author(s): Mami Yamazaki, Takashi Asano, Keiichi Mochida and Kazuki Saito
  • Organizer: A joint Meeting of TERPNET2013, Cost Action “Plant Engine”, Cost Action QualityFruit” and “EU-SmartCell”
  • Place of Presentation: Kolymbari, Crete, Greece
• [Presentation] Cloning and Characterization of Plant Lys Decarboxylase: Diversity and Molecular Evolution (2013)
  • Author(s): Somnuk Bunsupa, Hideki Ueno, Akira Maruyama, Madoka Yamashita, Akira Oikawa, Ryosuke, Sasaki, Kazuki Saito, Mami Yamazaki
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] Transcriptome mining of genes associated with quinolizidine alkaloids biosynthesis in Lupinus angustifolius (2013)
  • Author(s): Somnuk Bunsupa，山崎真巳，斉藤和季
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Presentation] 改変ストリクトシジン合成酵素を用いた非天然型カンプトテシン誘導体清算の試み (2013)
  • Author(s): 山崎真巳，山本天心，中林亮，斉藤和季
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Presentation] Deep-transcriptome analysis in Lupinus angustifolius: Mining and characterization of genes involved in quinolizidine alkaloid biosynthetic pathway (2012)
  • Author(s): Somnuk Bunsupa, Mimi Yamazaki, Kazuki Saito
  • Organizer: The International Conference of Natural Product Biosynthesis. 8th US-Japan Seminar: Enzymology・Structural Biology・Drug Discovery・Genome Mining
  • Place of Presentation: 淡路
• [Presentation] カンプトテシン生産特異的に発現する転写調節因子について (2012)
  • Author(s): 山﨑真巳，川原田美季，Udomsom Nirin，斉藤和季
  • Organizer: 第30回日本植物細胞分子生物学会（生駒）大会・シンポジウム
  • Place of Presentation: 生駒
• [Presentation] Funcional characterization of lysine decarboxylase from cadaverine-derived alkaloid-producing plants (2012)
  • Author(s): Bunsupa Somunk, Hideki Ueno, Maruyama Akira, Saito Kazuki, Yamazaki Mami
  • Organizer: 第30回日本植物細胞分子生物学会（生駒）大会・シンポジウム
  • Place of Presentation: 生駒
• [Presentation] 薬用植物の特異代謝研究におけるデータベースとオミクスの利用 (2012)
  • Author(s): 山﨑真巳
  • Organizer: 日本生薬学会第59回年会
  • Place of Presentation: 木更津
  • Invited
• [Presentation] カンプトテシンを高生産するチャボイナモリ毛状根特異的に発現するMYB様転写因子OpMYB1について (2012)
  • Author(s): 川原田美季，千葉基昌，斉藤和季，山﨑真巳
  • Organizer: 日本生薬学会第59回年会
  • Place of Presentation: 木更津
• [Presentation] Molecular clonig and characterization of lysine decarboxylase from Sophora flavescens and Lycopodium clavatum (2012)
  • Author(s): Somnuk Bunsupa, Hideki Ueno, Akira Maruyama, Kae Katayama, Kazuki Saito, Mami Yamazaki
  • Organizer: 日本生薬学会第59回年会
  • Place of Presentation: 木更津
• [Presentation] ERF Transcription Factors specifically expressed in hairy root of Ophiorrhiza pumila producing Campthothecin (2012)
  • Author(s): Nirin Udomsom, Miki Tokoro, Kazuki Saito, Mami Yamazaki
  • Organizer: 日本生薬学会第59回年会
  • Place of Presentation: 岡山
• [Book] Camptithecin Production and Biosynthesis in Plant Cell Culture. in “50 Years of Phytochemistry Research Recent Advances in Phytochemistry volume 43” (2013)
  • Author(s): Takashi Asano, Kazuki Saito and Mami Yamazaki
  • Total Pages: 159
  • Publisher: Springer Cham Heiderberg NewYork Dortrecht London
• [Remarks] 千葉大学大学院薬学研究院遺伝子資源応用研究室
  • URL: http://www.p.chiba-u.ac.jp/lab/idenshi/index.html
• [Remarks] 「苦い豆」の原因遺伝子を解明 ―薬用植物アルカロイドのバイオ生産や創薬に手がかり―
  • URL: http://www.p.chiba-u.ac.jp/pdf/20120313.pdf