Regulatory mechanism in metabolic allocation of nitrogen-containing compounds from central metabolisms to specialized metabolisms in plants
| Project/Area Number |
24570041
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Chiba University |
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Principal Investigator |
YAMAZAKI Mami 千葉大学, 薬学研究科(研究院), 准教授 (70222370)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | アルカロイド / アミノ酸 / リジン / オルニチン / トリプトファン / 特異代謝 / 二次代謝 / ポリアミン / リジン脱炭酸酵素 / 代謝アロケーション / アミン酸化酵素 / 代謝 / 脱炭酸酵素 |
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| Outline of Final Research Achievements |
Most of plant alkaloids are produced from amino acids. In this study, the molecular basis of metabolite allocation from amino acid metabolism (central metabolism) to alkaloid biosynthesis (specialized metabolism) was investigated. Quinolizidine alkaloids (QA) and lycopodium alkaloids (LA) are derived from lysine. is lysine decarboxylation. The cDNAs encoding lysine/ornithine decarboxylase (L/ODC) catalyzing the first step QA and LA biosynthesis were isolated from several species and characterized using recombinant protein expressed in E. coli. The enzymes from QA/LA-producing plants exhibited relatively higher lysine-decarboxylase activities comparing to those of other species. It is suggested that this difference leads the change in metabolic allocation from lysine metabolism to alkaloid biosynthesis.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Coupling deep transcriptome analysis with untargeted metabolic profiling in Ophiorrhiza pumila to further the understanding of the biosynthesis of the anti-cancer alkaloid camptothecin and anthraquinones2013
Author(s)
Mami Yamazaki, Keiichi Mochida, Takashi Asano, Ryo Nakabayashi, Motoaki Chiba, Udomson Nirin, Yasuyo Yamazaki, Dayan B. Goodenowe, Ushio Sankawa, Takuhiro Yoshida, Atsushi Toyoda, Yasushi Totoki, Yoshiyuki Sakaki, Elsa Góngora-Castill, C. Robin Buell, Tetsuya Sakurai and Kazuki Saito
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Journal Title
Plant Cell Physiol.
Volume: 54
Issue: 5
Pages: 686-696
DOI
NAID
Related Report
Peer Reviewed
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