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Molecular Mechanism of Chloroplast development Plasticity

Research Project

Project/Area Number 24570042
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe University of Tokyo

Principal Investigator

Masuda Tatsuru  東京大学, 総合文化研究科, 教授 (00242305)

Co-Investigator(Renkei-kenkyūsha) KOBAYASHI Koichi  東京大学, 大学院総合文化研究科, 助教 (40587945)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords葉緑体分化 / 転写因子 / 転写制御 / 光合成 / 共発現 / 植物ホルモン / 光シグナル / 葉緑体 / クロロフィル / 光化学系 / シロイヌナズナ / 根 / サイトカイニン
Outline of Final Research Achievements

In this study, we have studied the molecular mechanism of chloroplast development plasticity. We found that in excised Arabidopsis roots, chloroplast differentiation is activated through auxin- and cytokinin-signal transduction pathways. By excision of roots, suppression of chloroplast development by auxin, which is supplied from aerial tissues, is attenuated, while the cytokinin signal is activated by wounding, resulting in significant chloroplast development in excised roots. For this chloroplast development, coordinated transcriptional activation of nuclear-encoded photosynthesis genes is involved. We found transcription factors, HY5 and GLK, are actually involved in this activation. Interestingly, overexpressors of GLK showed pronounced induction of chloroplast development in intact roots and they showed higher photosynthetic competency when compared to that of wild type.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (26 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Invited: 2 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Expression analysis of transcription factors involved in chloroplast differentiation.2015

    • Author(s)
      Listiawan DA, Tanoue R, Kobayashi K, Masuda T
    • Journal Title

      Procedia Chemistry

      Volume: 14 Pages: 146-151

    • DOI

      10.1016/j.proche.2015.03.021

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation.2014

    • Author(s)
      Hori, Koichi, et al.
    • Journal Title

      Nature communications

      Volume: 5 Issue: 1 Pages: 1-9

    • DOI

      10.1038/ncomms4978

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcriptional regulation of thylakoid galactolipid biosynthesis coordinated with chlorophyll biosynthesis during the development of chloroplasts in Arabidopsis.2014

    • Author(s)
      Kobayashi, K., Fujii, S., Sasaki, D., Baba, S., Ohta, H., Masuda, T., and Wada, H.
    • Journal Title

      Frontiers of Plant Science

      Volume: 5 Pages: 272-272

    • DOI

      10.3389/fpls.2014.00272

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular phylogeny and intricate evolutionary history of the three isofunctional enzymes involved in the oxidation of protoporphyrinogen IX2014

    • Author(s)
      Kobayashi, K., Masuda, T., Tajima, N., Wada, H., Sato, N.
    • Journal Title

      Genome Biology and Evolution

      Volume: 6 Issue: 8 Pages: 2141-2155

    • DOI

      10.1093/gbe/evu170

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of galactolipid biosynthesis in coordinated development of photosynthetic complexes and thylakoid membranes during chloroplast biogenesis in Arabidopsis2013

    • Author(s)
      Kobayashi, K., T. Narise, K. Sonoike, H. Hashimoto, N. Sato, M. Kondo, M. Nishimura, M. Sato, K. Toyooka, K. Sugimoto, H. Wada, T. Masuda and H. Ohta
    • Journal Title

      Plant J.

      Volume: 73 Issue: 2 Pages: 250-261

    • DOI

      10.1111/tpj.12028

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photosynthesis of Root Chloroplasts Developed in Arabidopsis Lines Overexpressing GOLDEN2-LIKE Transcription Factors2013

    • Author(s)
      Kobayashi K, Sasaki D, Noguchi K, Fujinuma D, Komatsu H, Kobayashi M, Sato M, Toyooka K, Sugimoto K, Niyogi KK, Wada H, Masuda T
    • Journal Title

      Plant Cell Physiol

      Volume: 54(8) Issue: 8 Pages: 1365-1377

    • DOI

      10.1093/pcp/pct086

    • NAID

      10031195189

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Regulation of root greening by light and auxin/cytokinin signaling in Arabidopsis2012

    • Author(s)
      Kobayashi, K., Baba, S., Obayashi, T., Keranen, M., Aro, E.M., Fukaki, H., Ohta, H., Masuda T.
    • Journal Title

      Plant Cell

      Volume: 24 Pages: 1081-1095

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of the G-box element for regulation of chlorophyll biosynthesis in Arabidopsis root2012

    • Author(s)
      Kobayashi, K., Obayashi, T., Masuda, T.
    • Journal Title

      Plant Signal. Behavior

      Volume: 7 Issue: 8 Pages: 922-926

    • DOI

      10.4161/psb.20760

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Disrupting the bimolecular binding of the heme binding protein 5 (AtHBP5) with heme oxygenase I (HYI) leads to oxidative stress in Arabidopsis2012

    • Author(s)
      Lee H.-Y., Masuda, T., Buckhout, T. J.
    • Journal Title

      J. Exp. Bot.

      Volume: 63 Pages: 5967-5987

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evaluation ofunbound free heme in plant cells by differential acetone extraction2012

    • Author(s)
      Espinas NA, Kobayashi K, Takahashi S, Mochizuki N, Masuda T
    • Journal Title

      Plant Cell Physiol

      Volume: 53巻 Issue: 7 Pages: 1344-1354

    • DOI

      10.1093/pcp/pcs067

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 植物細胞におけるヘム代謝研究の新展開2012

    • Author(s)
      増田建
    • Journal Title

      光合成研究

      Volume: 64 Pages: 115-124

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Monogalactosyldiacylglycerol synthesis in the dark contributes to etiolation and chloroplast differentiation of Arabidopsis2016

    • Author(s)
      Fujii S, Kobayashi K, Masuda T, Wada H
    • Organizer
      第57回植物生理学会年会
    • Place of Presentation
      岩手大学(岩手県盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Chloroplast differentiation2014

    • Author(s)
      Masuda, T.
    • Organizer
      Academia Synica Symposium
    • Place of Presentation
      Taiwan
    • Year and Date
      2014-10-02
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Plastid differentiation controlled by coordinate transcriptional regulation of nuclear-encoded photosynthetic genes2014

    • Author(s)
      Masuda, T.
    • Organizer
      International Conference on Natural Sciences (ICONS 2014)
    • Place of Presentation
      Indonesia
    • Year and Date
      2014-09-24 – 2014-09-26
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 茎頂分裂組織特異的転写因子ISH1, ISH2の強調作業による根部での地上用器官の形成2014

    • Author(s)
      戸松創、花野滋、小林康一、近藤侑貴、別役重之、瀧田英司、山道桂子、太田啓之、細内敦、小澤馨史、長瀬隆弘、尾形善之、櫻井望、鈴木秀幸、増田建、福田裕穂、柴田大輔
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学 五福キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 車軸藻植物門クレビソルミディウムのゲノム解析と脂質代謝系の進化過程の解析2013

    • Author(s)
      堀孝一、丸山史人、藤澤貴智、富樫智章、山本希、円由香、下嶋美恵、瀬尾光範、佐藤修正、山田拓司、森宙史、田島直幸、森山崇、池内昌彦、渡邊麻衣、和田元、小林康一、斎藤勝和、増田建、他
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学 札幌キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] シロイヌナズナにおける2つのフェロキラターゼアイソフォームによるヘム分配機構の解析2013

    • Author(s)
      増田建、Nino, A. Espinas, 小林康一、佐藤康、高橋香織、田中亮一、望月伸悦
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学 札幌キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 傷害に応答した根の緑化に関わるサイトカイニンシグナル経路2013

    • Author(s)
      佐々木大地、岩瀬哲、杉本慶子、増田建、和田元、小林康一
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 車軸藻網クレブソルミディウムのゲノム解析と植物の陸上化要因の予測2013

    • Author(s)
      堀孝一、増田建、他
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Allocation of heme is differentially regulated by ferrochelatase isoforms in Arabidopsis cells2013

    • Author(s)
      Espinas, E., Kobayashi, K., Sato, Y., Mochizuki, N., Masuda, T.
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Photosynthesis in roots of Arabidopsis over expressing GLK2013

    • Author(s)
      Kobayashi, K., Sasaki, D., Noguchi, K., Fujinuma, D., Kobayashi, M., Sato, M., Toyooka, K., Sugimoto, K., Wada, H., Masuda, T.
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Book] 理系総合のための生命科学2013

    • Author(s)
      赤坂甲治、有田正規、石浦章一、井出利憲、伊藤元己、入村達郎、太田邦史、大矢禎一、堅田利明、児玉龍彦、笹川昇、佐藤健、佐藤直樹、嶋田正和、杉山崇隆、多田幸雄、立花政夫、塚谷裕一、坪井貴司、寺島一郎、豊島陽子、長野哲雄、野口航、福田裕穂、正木春彦、増田建、他
    • Total Pages
      333
    • Publisher
      羊土社
    • Related Report
      2013 Research-status Report
  • [Book] The Handbook of Porphyrin Science2012

    • Author(s)
      Kobayashi, K., Masuda, T.
    • Total Pages
      69
    • Publisher
      World Scientific Publishing
    • Related Report
      2012 Research-status Report
  • [Remarks] 植物代謝システム研究室(増田建研究室)

    • URL

      http://park.itc.u-tokyo.ac.jp/masuda_lab/Masuda_Laboratory/Welcome.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 増田研究室ホームページ

    • URL

      http://park.itc.u-tokyo.ac.jp/masuda_lab/Masuda_Laboratory/Welcome.html

    • Related Report
      2013 Research-status Report
  • [Remarks] Welcome

    • URL

      http://park.itc.u-tokyo.ac.jp/masuda_lab/Masuda_Laboratory/Welcome.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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