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Analysis of novel regulatory mechanisms for plant stem growth

Research Project

Project/Area Number 24570050
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNagoya University (2013-2014)
Nara Institute of Science and Technology (2012)

Principal Investigator

UCHIDA Naoyuki  名古屋大学, トランスフォーマティブ生命分子研究所, 特任准教授 (40467692)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords受容体 / リガンド / シロイヌナズナ
Outline of Final Research Achievements

In this project, we aimed to identify novel genes that regulate stem growth of plants. To that purpose, we used er mutant and epfl4 epfl6 mutnat. By comparing wild type, er and epfl4 epfl6 plants, it was revealed that cell proliferation decreases around the tip of stems in mutants compared with wild type. Then by performing comprehensive gene expression analysis, we identified a novel receptor gene whose expression level is down-regulated in mutants. Because there is a similar gene to the identified gene, we made the double mutant for these two genes. The double mutant showed defects in stem growth, indicating that these receptor genes play a important role in the regulation of stem growth. Thus, we achieved the goal of this project, that is, identification of novel genes that regulate stem growth.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (34 results)

All 2015 2014 2013 2012 Other

All Journal Article (9 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (25 results) (of which Invited: 2 results)

  • [Journal Article] Transcriptional, post-transcriptional and post-translational regulation of STM gene expression in Arabidopsis determine gene function in the shoot apex2015

    • Author(s)
      Aguilar-Martínez JA, Uchida N, Townsley B, West DA, Yanez A, Lynn N, Kimura S, Sinha N
    • Journal Title

      Plant Physiol.

      Volume: 167 Issue: 2 Pages: 424-442

    • DOI

      10.1104/pp.114.248625

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of EMS-induced Causal Mutations in Arabidopsis thaliana by Next-Generation Sequencing2014

    • Author(s)
      Uchida N, Sakamoto T, Tasaka M, Kurata T
    • Journal Title

      Methods Mol. Biol.

      Volume: 1062 Pages: 259-270

    • DOI

      10.1007/978-1-62703-580-4_14

    • ISBN
      9781627035798, 9781627035804
    • Related Report
      2013 Research-status Report
  • [Journal Article] Regulation of plant vascular stem cells by endodermis-derived EPFL-family peptide hormones and phloem-expressed ERECTA-family receptor kinases2013

    • Author(s)
      Uchida N, Tasaka M
    • Journal Title

      J. Exp. Bot.

      Volume: 64 Issue: 17 Pages: 5335-5343

    • DOI

      10.1093/jxb/ert196

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 植物が茎を伸ばす仕組みで働くスイッチの発見 ~植物の背丈を人為的に操る技術につながるか?2013

    • Author(s)
      打田 直行
    • Journal Title

      化学と生物

      Volume: 51 Pages: 588-589

    • Related Report
      2013 Research-status Report
  • [Journal Article] 花序の形態の制御で働くEPFL型ペプチドホルモンとその受容体2013

    • Author(s)
      打田 直行
    • Journal Title

      植物の生長調節

      Volume: 48 Pages: 67-72

    • Related Report
      2013 Research-status Report
  • [Journal Article] Regulation of inflorescence architecture by intertissue layer ligand-receptor communication between endodermis and phloem.2013

    • Author(s)
      Uchida N, Kakimoto T, 他
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 109 Issue: 16 Pages: 6337-6342

    • DOI

      10.1073/pnas.1117537109

    • NAID

      120005716671

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] ERECTA-family receptor kinases regulate stem cell homeostasis via buffering its cytokinin responsiveness in the shoot apical meristem2013

    • Author(s)
      Uchida N, Shimada M, Tasaka M
    • Journal Title

      Plant Cell Physiol.

      Volume: 54 Issue: 3 Pages: 343-351

    • DOI

      10.1093/pcp/pcs109

    • NAID

      10031163830

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Modulation of the balance between stem cell proliferation and consumption by ERECTA-family genes.2012

    • Author(s)
      Uchida N, Shimada M, Tasaka M.
    • Journal Title

      Plant Signal. Behav.

      Volume: 7 Issue: 11 Pages: 1506-1508

    • DOI

      10.4161/psb.22080

    • Related Report
      2012 Research-status Report
  • [Journal Article] Biological role of the two overlapping poly(A)-binding protein interacting motifs 2 (PAM2) of eukaryotic releasing factor eRF3 in mRNA decay.2012

    • Author(s)
      Osawa M, Hosoda N, Nakanishi T, Uchida N, Kimura T, Imai S, Machiyama A, Katada T, Hoshino S, Shimada I.
    • Journal Title

      RNA

      Volume: 18 Issue: 11 Pages: 1957-1967

    • DOI

      10.1261/rna.035311.112

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ERECTA-family receptor kinases regulate secondary growth in hypocotyls of Arabidopsis thaliana2015

    • Author(s)
      池松朱夏, 田坂昌生, 鳥居啓子, 打田直行
    • Organizer
      日本植物生理学会年会第56回大会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] ERECTA-family receptors alter WUSCHEL-dependency and cytokinin responsiveness in stem cell maintenance2015

    • Author(s)
      木村友香, 鳥居啓子, 田坂昌生, 打田直行
    • Organizer
      日本植物生理学会年会第56回大会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Analysis of CLE peptide genes in Marchantia2015

    • Author(s)
      平川有宇樹, 田畑亮, 河内孝之, 打田直行, 澤進一郎, John Bowman
    • Organizer
      日本植物生理学会年会第56回大会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Analysis of de novo SNP spectrum in uni-1D mutant2015

    • Author(s)
      6)森明子, 小川与比古, 猪狩和成, 森田(寺尾)美代, 田坂昌生, 打田直行
    • Organizer
      日本植物生理学会年会第56回大会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Role of CLE peptide signaling in Marchantia development2014

    • Author(s)
      Yuki Hirakawa, Ryo Tabata, Kimitsune Ishizaki, Takayuki Kohchi, Naoyuki Uchida, John Bowman, Shinichiro Sawa
    • Organizer
      Marchantia Workshop 2014
    • Place of Presentation
      神戸
    • Year and Date
      2014-12-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Regulation of plant stem cell homeostasis via coordination among multiple signaling pathways2014

    • Author(s)
      Uchida N, Kimura Y, Torii KU, Tasaka M
    • Organizer
      The 38th Naito Conference on Molecule-based biological systems
    • Place of Presentation
      北海道
    • Year and Date
      2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ERECTA受容体ファミリーがコーディネートする発生・成長シグナリング2014

    • Author(s)
      木村友香, 池松朱夏, 松原健太, 爲重才覚, 平川有宇樹, 田坂昌生, 鳥居啓子, 打田直行
    • Organizer
      日本植物学会大78回大会
    • Place of Presentation
      神奈川
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 陸上植物におけるCLE 遺伝子の機能解析2014

    • Author(s)
      平川 有宇樹,Bowman John,打田 直行
    • Organizer
      日本植物学会大78回大会
    • Place of Presentation
      神奈川
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] ERECTA ファミリー受容体とペプチドリガンドによるオーキシン応答パターンの制御2014

    • Author(s)
      爲重 才覚,岡本 智史,Lee Jin Suk,相田 光宏,田坂 昌生,Torii Keiko,打田 直行
    • Organizer
      日本植物学会大78回大会
    • Place of Presentation
      神奈川
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Regulation of plant growth and morphogenesis by receptor signaling2014

    • Author(s)
      Naoyuki Uchida
    • Organizer
      The 2nd International Symposium on Transformative Bio-Molecules
    • Place of Presentation
      名古屋
    • Year and Date
      2014-05-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 植物の背丈をコントロールするスイッチの発見

    • Author(s)
      打田 直行
    • Organizer
      豊秋奨学会平成25年度国際学生交流会・同窓会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Mechanisms for diversification of R genes revealed by analysis of uni-1D mutant

    • Author(s)
      打田直行, 小川与比古, 森明子, 猪狩和成, 森田(寺尾)美代, 田坂昌生
    • Organizer
      日本植物生理学会年会第55回大会
    • Place of Presentation
      富山
    • Related Report
      2013 Research-status Report
  • [Presentation] Regulation of inflorescence stem growth by the pair of peptide hormones EPFL4/6 and their receptor ERECTA

    • Author(s)
      野中菜乃美、池松朱夏、田坂昌生、打田直行
    • Organizer
      日本植物生理学会年会第55回大会
    • Place of Presentation
      富山
    • Related Report
      2013 Research-status Report
  • [Presentation] Regulation of vascular development of Arabidopsis hycopotyls by ERECTA-family receptor kinases during secondary growth

    • Author(s)
      池松朱夏、野田口理孝、東山哲也、鳥居啓子、田坂昌生、打田直行
    • Organizer
      日本植物生理学会年会第55回大会
    • Place of Presentation
      富山
    • Related Report
      2013 Research-status Report
  • [Presentation] Analysis of signaling crosstalks for vascular growth in Arabidopsis hypocotyls

    • Author(s)
      松原健太、池松朱夏、平川有宇樹、東山哲也、鳥居啓子、田坂昌生、打田直行
    • Organizer
      日本植物生理学会年会第55回大会
    • Place of Presentation
      富山
    • Related Report
      2013 Research-status Report
  • [Presentation] Novel mechanism for stem cell maintenance suggested by analysis of ERECTA-family receptor kinase genes

    • Author(s)
      木村友香、田坂昌生、打田直行
    • Organizer
      日本植物生理学会年会第55回大会
    • Place of Presentation
      富山
    • Related Report
      2013 Research-status Report
  • [Presentation] Functional analysis of a Marchantia CLE gene

    • Author(s)
      平川有宇樹、田畑亮、本田紘章、打田直行、河内孝之、澤進一郎、John Bowman
    • Organizer
      日本植物生理学会年会第55回大会
    • Place of Presentation
      富山
    • Related Report
      2013 Research-status Report
  • [Presentation] A novel model system to study the rapid diversification of R genes.

    • Author(s)
      Uchida N, Lee JS, Torii KU, Tasaka M
    • Organizer
      GENETIC STABILITY & CHANGE: Genome maintenance mechanisms in plants
    • Place of Presentation
      Roscoff, France
    • Related Report
      2012 Research-status Report
  • [Presentation] Regulation of inflorescence architecture by intertissue layer ligand-receptor communication between endodermis and phloem.

    • Author(s)
      Uchida N
    • Organizer
      Plant Vascular Development 2012
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2012 Research-status Report
  • [Presentation] Regulation of inflorescence architecture by novel peptide hormones via communication between endodermis and phloem

    • Author(s)
      Uchida N
    • Organizer
      The 23rd International Conference on Arabidopsis Research
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2012 Research-status Report
  • [Presentation] Regulation of plant vascular stem cells by ERECTA-family receptor kinases

    • Author(s)
      Uchida N
    • Organizer
      The 1st European Workshop on Peptide Signalling in Plants
    • Place of Presentation
      Oslo, Norway
    • Related Report
      2012 Research-status Report
  • [Presentation] Regulation of plant vascular stem cells by ERECTA-family receptor kinases

    • Author(s)
      打田直行, 田坂昌生
    • Organizer
      日本植物生理学会年会第54回大会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Research-status Report
  • [Presentation] Regulation of stem cell homeostasis by ERECTA-family receptor kinases.

    • Author(s)
      木村友香、島田昌典、田坂昌生、打田直行
    • Organizer
      日本植物生理学会年会第54回大会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Research-status Report
  • [Presentation] Novel ligand-receptor pair for leaf tooth formation

    • Author(s)
      岡本智史、相田 光宏、田坂昌生、打田直行
    • Organizer
      日本植物生理学会年会第54回大会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Research-status Report
  • [Presentation] Regulation of hypocotyl vasculatures by ERECTA-family receptor kinases during secondary growth.

    • Author(s)
      池松朱夏、田坂昌生、打田直行
    • Organizer
      日本植物生理学会年会第54回大会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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