| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We investigated the regulatory mechanism of RHD3, a fusogen of endoplasmic reticulum (ER) membrane. The fusion of ER membrane isolated from Arabidopsis seedling and cultured cell, and the oligomerization of RHD3 were enhanced by the treatment with protein kinases. The serine residues in the C-terminus serine cluster of RHD3 were phosphorylated. The membrane fusion enhancement did not occur with isolated ER from seedlings expressing the RHD3 with disrupted phosphorylation sites in the serine cluster. These results indicated that the phosphorylation of RHD3 regulates the ER membrane fusion.
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