| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Protein phosphatase 2C (PP2C) plays a major role in the negative regulation of signaling pathway for a plant hormone abscisic acid (ABA). Disruption of PP2C genes in the moss Physcomitrella patens by gene targeting resulted in acquisition of extreme tolerance to osmostresses such as desiccation and freezing. On the other hand, the PP2C disruptant stayed green even after prolonged culture in the dark, while the wild type completely etiolated. To understand the crosstalk between ABA signaling and light signaling in the moss, we performed molecular analysis using various ABA-related mutants of P. patens. These analyses indicated that ABA plays a negative role in etiolation of chloroplasts in the dark, and further suggested that chlorophyll biosynthesis pathway is activated in the dark-grown PP2C disruptant and PP2C is involved in autophagy machinery.
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