| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Understanding the association between stress resistance and 5-deoxyflavonoid metabolism of Acacia mangium, a stress-resistant leguminous tree, has been attempted by the following investigations. cDNA cloning and functional analysis of enzyme genes involved in the flavonoid biosynthesis revealed that the key enzymes were able to catalyze 5-deoxyflavonoids as well as 5-hydroxyflavonoids as substrate. Caffeic acid was identified among the metabolites produced by suspension-cultured cells of A. mangium in response to low pH and aluminum stresses. A lot of up-regulated genes under the acid stress were detected. The cloned genes were used to produce transgenic plants that enhance or suppress the production of 5-deoxyflavonoids.
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