Elucidation of ribosome-mediated developmental mechanisms in plants

• Project/Area Number: 24570060
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Rikkyo University
• Principal Investigator: HORIGUCHI Gorou 立教大学, 理学部, 准教授 (70342847)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: リボソーム / AS2 / RPL4D / NAC転写因子 / RING finger protein / プロテオスタシス / シロイヌナズナ / 背腹性 / as2 / 転写因子 / 葉 / 葉の向背軸 / NAC型転写因子 / 翻訳制御

Outline of Final Research Achievements

Development of flat leaf structure is dependent on the appropriate adaxial-abaxial patterning. as2 rpl4d mutant has a leaf polarity defect in which leaves are strongly abaxialized. Isolation of suppressor mutants for as2 rpl4d, their phenotypic characterizations, and cloning of their responsible genes revealed following mechanisms. Strong leaf abaxialization is induced by upregulation of a NAC transcription factor gene, SUZAKU1 (SZK1). This upregulation is mediated by reduced mRNA level of RPL4D yet the abaxialization phenotype requires the presence of aberrant RPL4D protein. The SZK1 induction is also dependent on a RING finger protein, SZK2, and a ribosomal protein RPL12B.

Research Products

• [Journal Article] Nitrogen dioxide regulates organ growth by controlling cell proliferation and enlargement in Arabidopsis (2013)
  • Author(s): Takahashi, M., Furuhashi, T., Ishikawa, N., Horiguchi, G., Sakamoto, A., Tsukaya, H., Moikawa, H.
  • Journal Title: New Phytol. Volume: 201 Issue: 4 Pages: 1304-1315
  • DOI: 10.1111/nph.12609
  • Peer Reviewed
• [Journal Article] Class III compensation, represented by KRP2 overexpression, depends on V-ATPase activity in proliferative cells. (2013)
  • Author(s): Ferjani A, Ishikawa K, Asaoka M, Ishida M, Horiguchi G, and Tsukaya H.
  • Journal Title: Plant Sig. Behav Volume: - Issue: 11 Pages: e27204-e27204
  • DOI: 10.4161/psb.27204
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Promotion of chloroplast proliferation upon enhanced post-mitotic cell expansion in leaves. (2013)
  • Author(s): Kawade K, Horiguchi G, Ishikawa N, Hirai Yokota M, and Tsukaya H
  • Journal Title: BMC Plant Biol. Volume: 13 Issue: 1 Pages: 143-143
  • DOI: 10.1186/1471-2229-13-143
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Enhanced cell expansion in KRP2 overexpressor is mediated through increased V-ATPase activity. (2013)
  • Author(s): Ferjani, A., Ishikawa, K., Asaoka, M., Ishida, M., Horiguchi, G., Maeshima, M. and Tsukaya, H.
  • Journal Title: Plant Cell Physiology Volume: 54 Issue: 12 Pages: 1989-1998
  • DOI: 10.1093/pcp/pct138
  • Peer Reviewed
• [Journal Article] The ATM-dependent DNA damage response acts as an upstream trigger for compensation in the fas1 mutation during Arabidopsis leaf development (2013)
  • Author(s): Hisagana T, Ferjani A, Horiguchi G, Ishikawa N, Fujikura U, Kubo M, Demura T, Fukuda H, Ishida T, Sugimoto K, Tsukaya H
  • Journal Title: Plant Physiol. Volume: 162(2) Issue: 2 Pages: 831-841
  • DOI: 10.1104/pp.113.216796
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] ANGUSTIFOLIA3 signaling coordinates proliferation between clonally distinct cells in leaves. (2013)
  • Author(s): Kawade K, Horiguchi G, Usami T, Yokota M, and Tsukaya H.
  • Journal Title: Curr. Biol. Volume: 23 Issue: 9 Pages: 788-792
  • DOI: 10.1016/j.cub.2013.03.044
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] How do 'housekeeping genes' control organogenesis? -unexpected new findings on the role of housekeeping genes in cell and organ differentiation. (2013)
  • Author(s): Hirokazu Tsukaya
  • Journal Title: Journal of Plant Research Volume: 126 Issue: 1 Pages: 3-15
  • DOI: 10.1007/s10265-012-0518-2
  • Peer Reviewed
• [Journal Article] Ribosomes and translation in plant developmental control (2012)
  • Author(s): Horiguchi, G., Van Lijsebettens, M., Candela, H., Micol, J.L., Tsukaya, H.
  • Journal Title: Plant Sci. Volume: 191-192 Pages: 24-34
  • DOI: 10.1016/j.plantsci.2012.04.008
  • Peer Reviewed
• [Journal Article] Stable establishment of cotyledon identity during embryogenesis in Arabidopsis by ANGUSTIFOLIA3 and HANABA TARANU (2012)
  • Author(s): Kanei, M., Horiguchi, G., Tsukaya, H.
  • Journal Title: Development Volume: 139 Issue: 13 Pages: 2436-2446
  • DOI: 10.1242/dev.081547
  • Peer Reviewed
• [Journal Article] Berberine enhances defects in the establishment of leaf polarity in asymmetric leaves2 and asymmetric leaves1 of Arabidopsis thaliana (2012)
  • Author(s): Nakagawa A, Takahashi H, Kojima S, Sato N, Ohga K, Cha BY, Woo J-T, Nagai K, Horiguchi G, Tsukaya H, Machida Y and Machida C
  • Journal Title: Plant Mol Biol Volume: 79 Issue: 6 Pages: 569-581
  • DOI: 10.1007/s11103-012-9929-7
  • Peer Reviewed
• [Presentation] as2 rpl4d背景での葉の裏側化を抑制する変異体の遺伝学および発生学的解析 (2015)
  • Author(s): 高原正裕、塚谷裕一、堀口吾朗
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 根のプラスチドリボソームの損傷は側根の発生異常を引き起こす (2015)
  • Author(s): 中田 未友希、塚谷 裕一、堀口 吾朗
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 原核型リボソーム阻害剤がシロイヌナズナの根の発生に与える影響 (2014)
  • Author(s): 中田未友希、塚谷裕一、堀口吾朗
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学 (神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] リボソームタンパク質変異体で過剰発現するSZK1の解析 (2014)
  • Author(s): 井上幹人、中田未友希、塚谷裕一、堀口吾朗
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学 (神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] リボソームタンパク質RPL4の質と量が葉の背腹性に及ぼす効果の解析 (2014)
  • Author(s): 増田英典、塚谷裕一、堀口吾朗
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学 (神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] SUZAKU1, a NAC-domain transcription factor gene promote leaf abaxialization in response to as2-enhancer mutations (2014)
  • Author(s): Horiguchi, G., Inoue, M., Masuda H., Nakata, M., Tsukaya, H.
  • Organizer: 25th International Conference on Arabidopsis Research
  • Place of Presentation: Vancouver (Canada)
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] ボソームタンパク質RPL4DのGFP標識とその機能性についての解析 (2014)
  • Author(s): 堀口吾朗、中田未友希、塚谷裕一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学、富山県、富山市
• [Presentation] 根の発生におけるプラスチドタンパク質RFC3の機能解析 (2014)
  • Author(s): 中田未友希、塚谷裕一、堀口吾朗
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学、富山県、富山市
• [Presentation] an3依存的補償作用に関与する外的因子の検討 (2014)
  • Author(s): 江崎 和音、川出 健介、堀口吾朗、塚谷 裕一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学、富山県、富山市
• [Presentation] シロイヌナズナのWD40リピートタンパク質をコードするOLIGOCELLULA1は異形葉性を通じて葉のサイズ制御に関わる (2013)
  • Author(s): 篠塚 奈々絵、平方 智大、藤倉 潮、出村 拓、塚谷 裕一、堀口 吾朗
  • Organizer: 日本植物学会
  • Place of Presentation: 北海道大学、北海道、札幌市
• [Presentation] NAC型転写因子をコードするSZK1遺伝子の高発現が葉の背腹性に及ぼす効果の解析 (2013)
  • Author(s): 堀口吾朗、塚谷裕一
  • Organizer: 日本植物学会
  • Place of Presentation: 北海道大学、北海道、札幌市
• [Presentation] シロイヌナズナの器官サイズ異常変異株little princeはミトコンドリアnad6 mRNAのエディティング異常を示す (2013)
  • Author(s): 石橋幸大、濱田ゆかり、中村崇裕、塚谷裕一、堀口吾朗
  • Organizer: 日本植物形態学会
  • Place of Presentation: 北海道大学、北海道、札幌市
• [Presentation] Mutations in a NAC-domain transcription factor gene, SUZAKU1, suppress leaf abaxialization caused by the defects of ribosomal proteins (2013)
  • Author(s): Gorou Horiguchi, Hiroki Shimada, Tatsuya Watanabe, Hirokazu Tsukaya3
  • Organizer: FASEB Conference: Mechanisms in Plant Development
  • Place of Presentation: Vermont academy, Saxtons River, Vermont, USA
• [Presentation] NAC転写因子の機能欠損によるas2 rpl4d が示す葉の背軸化の抑制 (2013)
  • Author(s): 堀口吾朗、島田浩貴、渡辺達矢、大林 祝、杉山宗隆、塚谷裕一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] リボソーム機能の不全はNAC転写因子ANAC082を介して発生過程に影響する (2013)
  • Author(s): 大林 祝、松村葉子、堀口吾朗、塚谷裕一、町田泰則、杉山宗隆
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] rpl4d as2 の葉が背軸化 する表現型を抑圧する szk1-D 変異株の解析 (2012)
  • Author(s): 島田浩貴、渡辺達矢、大林 祝、杉山宗隆、塚谷裕一 、堀口 吾朗
  • Organizer: 日本植物学会
  • Place of Presentation: 兵庫県立大学
• [Presentation] rpl4dが示す花序形態異常の解析 (2012)
  • Author(s): 尾内紀之、塚谷裕一、堀口吾朗
  • Organizer: 日本植物形態学会
  • Place of Presentation: 兵庫県立大学
• [Remarks] 立教大学理学部生命理学科堀口研ホームページ
  • URL: http://www2.rikkyo.ac.jp/web/horiguchi/plant1/home.html
• [Remarks] 立教大学理学部堀口研究室ホームページ
  • URL: http://www2.rikkyo.ac.jp/web/horiguchi/plant/home.html
• [Remarks] 堀口研究室ホームページ
  • URL: http://www2.rikkyo.ac.jp/web/horiguchi/plant11/home.html