Mechanisms of epigenetic regulation maintained by AS2 in leaf development of Arabidopsis thaliana
Project/Area Number |
24570061
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Chubu University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
KOJIMA Shoko 中部大学, 応用生物学部, 講師 (10340209)
TAKAHASHI Hiro 千葉大学大学院, 園芸学研究科, 准教授 (30454367)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | 植物 / 発生・分化 / 遺伝子 / 発現制御 / マイクロアレイ / エピジェネティック制御 / DNAメチル化 / ケミカルバイオロジー / エピジェネテック制御 |
Outline of Final Research Achievements |
Leaves develop as flat lateral organs from a shoot apical meristem. Initially, a group of cells is patterned along the proximal-distal axis and then the adaxial-abaxial axis is established, which is crucial for further leaf development. Subsequent cell proliferation along the medial-lateral axis results in flat and symmetric leaves. However, molecular mechanisms are still unknown. We reported that the ASYMMETRIC LEAVES1 (AS1)-AS2 complex represses AUXIN RESPONSE FACTOR3 (ARF3) directly and epigenetically, and indirectly represses ARF3 by PTGS. We found that TOP1α was involved in the AS1-AS2 repression of ARF3. Furthermore, bioinformatic analysis and molecular genetic analysis indicated that expression of Kip-related protein5 (KRP5) (CDK inhibitor) was controlled by AS1-AS2 through ARF3 functions. These results suggest that AS1-AS2 represses KRP5 for regulating cell division in the medial-lateral axis, and it might result in formation of flat and symmetric lamina.
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Report
(4 results)
Research Products
(66 results)
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[Journal Article] Berberine enhances defects in the establishment of leaf polarity in asymmetric leaves2 and asymmetric leaves1 of Arabidopsis thaliana2012
Author(s)
Nakagawa A, Takahashi H, Kojima S, Sato N, Ohga K, Cha BY, Woo J-T, Nagai K, Horiguchi G, Tsukaya H, Machida Y and Machida C
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Journal Title
Plant Mol Biol
Volume: 79
Issue: 6
Pages: 569-581
DOI
Related Report
Peer Reviewed
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[Presentation] The AS1-AS2-ETT pathway regulates cytokinin synthesis and cell division.2013
Author(s)
Machida C., Takahashi H., Iwasaki M., Nakagawa A., Ishibashi N., Eshed Y., Kojima S., Machida Y.
Organizer
FASEB Science Research Conferences (SRC) for 2013, Mechanisms in Plant Development
Place of Presentation
Vermont Academy (Saxtons River, Vermont, USA)
Related Report
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[Presentation] Identification of downstream targets of ASYMMETRIC LEAVES2 and its modifiers during leaf development in Arabidopsis thaliana.2013
Author(s)
Kojima S., Takahashi H., Ishibashi N., Handayani A., Matsumura Y., Prananingrum P., Machida Y. and Machida C.2. Kojima S., Takahashi H., Ishibashi N., Handayani A., Matsumura Y., Prananingrum P., Machida Y. and Machida C.
Organizer
FASEB Science Research Conferences (SRC) for 2013, Mechanisms in Plant Development
Place of Presentation
Vermont Academy (Saxtons River, Vermont, USA)
Related Report
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