Analysis of the "vacuolar bulb", -its biogenesis, function, and molecular basis-
Project/Area Number |
24570064
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | The University of Tokyo |
Principal Investigator |
CHIEKO Saito 東京大学, 大学院理学系研究科(理学部), 特任准教授 (10321762)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 液胞 / 微小管 / 液胞膜 / シロイヌナズナ / 透過電子顕微鏡 / 共焦点レーザー顕微鏡 / ライブイメージング / 遺伝学 |
Outline of Final Research Achievements |
Plant vacuole is a single-membrane-bound organelle, that occupy more than 90% volume in matured cell. Although the vacuole is often illustrated in many textbooks as a water-balloon-like shape with smooth surface, it has complex three-dimensional configuration actually. Typical examples of such complex shape in vacuolar membrane are transvacuolar strands or "(vacuolar) bulbs" we previously found. I analyzed the biosynthesis pathway of this "(vacuolar) bulb" by transmission electron microscopy using rapid-freeze fixation/freeze substitution. In wild-type cotyledon, the engulfment of the small electron lucent vacuole by large electron dense vacuole is often observed. Two mutants, sgr2-1 and zig-1/vti11, that we previously reported as "(vacuolar) bulb-less" mutants, lacked this engulfment event. These results suggest that the engulfment of small vacuole by big vacuole will make spherical shape and two membranes closely attached, those are typical characters of "(vacuolar) bulbs".
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Report
(4 results)
Research Products
(15 results)
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[Presentation] 液胞と bulb の形態に異 常をきたす変異体候補 #64 の原因遺伝子同定と 解析2012
Author(s)
齊藤 知恵子 , 木内 玲 子 , 中野 雄司, 楢本 悟 史, 植村 知博, 粟井 千 絵, 山上 あゆみ, 坂本 智昭, 倉田 哲也, 安部 弘 上田 貴志, 中野 明 彦
Organizer
日本植物学会第76回大会
Place of Presentation
兵庫県立大学
Year and Date
20120915-20120917
Related Report
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[Book] Atlas of Plant Cell Structure2014
Author(s)
Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y. (Eds.)
Total Pages
228
Publisher
Springer
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