Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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Outline of Final Research Achievements |
Kisspeptin plays pivotal roles in reproduction in mammals. However, its functions in non-mammalian species remain enigmatic. I therefore analyzed signal transduction of medaka (Olyzias latipes) GPR74, which reportedly receives kisspeptin in human, and GPR147, which share common ligands (NPFF/RFRP) with GPR74. At low concentrations, NPFF or RFRP reduced forskolin-induced CRE-luciferase activity in HEK293T cells expressing GPR74/GPR147 . However, at higher concentrations, NPFF/RFRP increased CRE-luciferase activity in these cells. Such a concentration-dependent biphasic responsiveness of GPR74/GPR147 to ligands may suggest that medaka GPR74 and GPR147 have an intrinsic ability of maintaining the cellular response within a certain range.
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