Central regulatory system of reproduction by neuropeptides including kisspeptin.
Project/Area Number |
24570067
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Morphology/Structure
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Research Institution | St. Marianna University School of Medicine (2015) The University of Tokyo (2012-2014) |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | キスペプチン / ニューロペプチドFF / NPFF / GPR74 / GPR147 / kiss1 / kiss2 / メダカ / NPFF受容体 / GnRH / 自己・傍分泌 / シグナル伝達 / NPFFR2 / GPF74 / プロテインキナーゼA / 終神経 / GPR54 |
Outline of Final Research Achievements |
Kisspeptin plays pivotal roles in reproduction in mammals. However, its functions in non-mammalian species remain enigmatic. I therefore analyzed signal transduction of medaka (Olyzias latipes) GPR74, which reportedly receives kisspeptin in human, and GPR147, which share common ligands (NPFF/RFRP) with GPR74. At low concentrations, NPFF or RFRP reduced forskolin-induced CRE-luciferase activity in HEK293T cells expressing GPR74/GPR147 . However, at higher concentrations, NPFF/RFRP increased CRE-luciferase activity in these cells. Such a concentration-dependent biphasic responsiveness of GPR74/GPR147 to ligands may suggest that medaka GPR74 and GPR147 have an intrinsic ability of maintaining the cellular response within a certain range.
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Report
(5 results)
Research Products
(12 results)
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[Presentation] Morphological analysis of the early development of GnRH neuron systems2013
Author(s)
Takahashi, A., Islam, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
Organizer
The 43th Annual Meeting of theSociety for Neuroscience
Place of Presentation
San Diego, United States
Related Report
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